Microarrays: Preparation, Microfluidics, Detection Methods, and Biological Applications

ISBN-13: 9781441924902 / Angielski / Miękka / 2010 / 356 str.

Kilian Dill; Robin Liu; Piotr Grodzinsky

Opis

Combinatorial chemistry is used to find materials that form sensor microarrays. This book discusses the fundamentals, and then proceeds to the many applications of microarrays, from measuring gene expression (DNA microarrays) to protein-protein interactions, peptide chemistry, carbodhydrate chemistry, electrochemical detection, and microfluidics.

Recenzje

From the reviews:

"Microarray technology is not without its challenges and limitations ... this book highlight novel approaches that may address some of those issues and offer suggestions of new strategies on the horizon. ... I found many of the topics in the book to be interesting ... . it has a considerable amount of useful material for someone who ... is looking for a broader perspective on applications. It will also be useful to those focused on electrochemical detection and microfluidics in microarrays." (Peter Wentzell, Journal of the American Chemical Society, Vol. 131 (36), 2009)

Spis treści

Overview and New Detection Method.- The Current Status of DNA Microarrays.- Electrochemical Detection on Microarrays.- Fluidic Manipulation and Microarrays.- Fully Integrated Microfluidic Device for Direct Sample-to-Answer Genetic Analysis.- Integrated Microfluidic Devices for Automated Microarray-Based Gene Expression and Genotyping Analysis.- Statistical Data Evaluation on Microarrays.- Intensity Concentration Relationships for Electrochemical Detection.- Applications.- Genotyping Arrays.- Peptide-Based Microarray.- Protein Microarrays for the Detection of Biothreats.- Photo-Generation of Carbohydrate Microarrays.- Expression Profiling Using Microfluidic Living Cell Arrays.- New Approaches to the Synthesis of Addressable Microarray Molecular Libraries.- eSensor®A Microarray Technology Based on Electrochemical Detection of Nucleic Acids and Its Application to Cystic Fibrosis Carrier Screening.- Future Improvements in Microarray Sensing.- Use of Redox Enzymes for the Electrochemical Detection of Sequence-Specific DNA and Immunochemical Entities.- Biochip Platforms for DNA Diagnostics.- MagArray Biochips for Protein and DNA Detection with Magnetic Nanotags: Design, Experiment, and Signal-to-Noise Ratio.- Bar Coding Platforms for Nucleic Acid and Protein Detection.- Electrochemical Nanoparticle-Based Sensors.

Blurb

The area of microarrays has evolved from genomics, and has been applied to proteomics and single cell studies as well. The applications of microarrays benefit diverse fields ranging from fundamental systems biology to practical diagnostic tests. This book provides a critical analysis of the applications of microarrays in several key areas of bioanalytical research including DNA studies, peptide arrays, protein arrays, combinatorial chemistry arrays, cell-based arrays, and glycoarrays. Further progress in microarrays is being spurred by the advances in micro- and nano-fabrication technologies, modern molecular biology, and data mining. This book is designed to be a useful source of fundamental and current information for microarray research practitioners and for those who are just entering this field.