Microarray Technology: Methods and Applications

ISBN-13: 9781493931354 / Angielski / Twarda / 2015 / 294 str.

Paul Li; Abootaleb Sedighi; Lin Wang

Opis

This volume provides updates of this established field in both methods and applications, as well as advances in applications of the microarray method to biomarkers such as DNAs, RNAs, proteins, glycans and whole cells. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Microarray Technology: Methods and Applications aims to ensure successful results in the further study of this vital field.

Spis treści

Part I: Introduction

 

1. Overview of Microarray Technology

Paul C. H. Li

 

Part II: Advances on Techniques in Microarray Technology

 

2. Surface Functionalization for Immobilization of Probes on Microarrays

C. Desmet and C.A. Marquette

 

3. The “clickable” Method for Oligonucleotide Immobilization onto Azide-Functionalized Microarrays

Tomasz Ratajczak, Barbara Uszczyńska, Emilia Frydrych-Tomczak, and Marcin K. Chmielewski

 

4. Microarray Developed on Plastic Substrates

María-José Bañuls; Sergi B. Morais, Luis A. Tortajada-Genaro, and Ángel Maquieira

 

5. Detection and Quantification of Micrornas by Ligase-Assisted Sandwich Hybridization on a Microarray

Ryo Iizuka, Taro Ueno, and Takashi Funatsu

 

6. Probe Design Strategies for Oligonucleotide Microarrays

Nicolas Parisot, Eric Peyretaillade, Eric Dugat-Bony, Jérémie Denonfoux, Antoine Mahul, and Pierre Peyret

 

PART III: Applications of Microarray Technology to Nucleic Acid Assays

 

7. Analyzing Illumina Gene Expression Microarray Data Obtained from Human Whole Blood Cell and Blood Monocyte Samples

Alexander Teumer, Claudia Schurmann, Arne Schillert, Katharina Schramm, Andreas Ziegler, and Holger Prokisch

 

8. Microarray Technology and its Applications for Detecting Plasma microRNA Biomarkers in Digestive Tract Cancers

Hirotaka Konishi, Daisuke Ichikawa, Tomohiro Arita, and Eigo Otsuji

 

9.  Application of DNA Microarray to Clinical Diagnostics

Ankita Patel and Sau W. Cheung

 

10. High-throughput DNA Array for SNP Detection of KRAS Gene using a Centrifugal Microfluidic Device

Abootaleb Sedighi and Paul C. H. Li

 

11. Single Nucleotide Polymorphisms as Genomic Markers for High-Throughput Pharmacogenomic Studies

Annalisa Lonetti, Maria Chiara Fontana, Giovanni Martinelli, and Ilaria Iacobucci

 

12. DNA Microarray-Based Diagnostics

Mahsa Gharibi Marzancola, Abootaleb Sedighi, and Paul C. H. Li

 

Part IV: Applications of Microarray Technology to Other Biological Assays

 

13. High-throughput Screening of Substrate Specificity for Protein Tyrosine Phosphatases (PTPs) On Phosphopeptide Microarrays

Liqian Gao, Su Seong Lee,  Jun Chen, Hongyan Sun, Yuliang Zhao, Zhifang Chai, and Yi Hu

 

14. Nanotechnology in the Fabrication of Protein Microarrays

Manuel Fuentes, Paula Díez, and  Juan Casado-Vela

 

15. Epitope Mapping using Peptide Microarray in Autoantibody Profiling

Sebastian Henkel, Robert Wellhausen, Dirk Woitalla, Katrin Marcus, and Caroline May

 

16. Preparation of Glycan Arrays using Pyridylaminated Glycans

Shin-ichi Nakakita and Jun Hirabayashi

 

17. Competitive Immunoassays Using Antigen Microarrays

Zhaowei Zhang, Weihua Hu, Qi Zhang, Peiwu Li, and Changming Li

 

18. Parallel Syntheses of Peptides on Teflon-patterned paper arrays (SyntArrays)

Frédérique Deiss, Yang Yang, and Ratmir Derda

 

19. Cell Microarrays for Biomedical Applications

Mario Rothbauer, David Wartmann, Verena Charwat, and Peter Ertl