Microarray Innovations: Technology and Experimentation

ISBN-13: 9780367385811 / Angielski / Miękka / 2019 / 360 str.

Gary Hardiman

Opis

Microarray technologies and applications have evolved steadily with increasing content and better analytical methodologies. This compendium represents a cross section of the past five years of microarray methods and applications and their use in drug discovery and biomedical research. Expert contributors cover improvements in automation, new substrates for printing arrays, platform comparisons and contrasts, experimental design, data normalization and mining schemes, and epigenomic array studies. It also reviews electronic microarrays, comparative genomic hybridization, microRNA arrays, mutational analyses, clinical diagnostic arrays, protein arrays, and neuroscience applications.

Spis treści

Introduction to Gene Expression Profiling with DNA Microarray Technology. Introduction to Large-Scale Gene Expression Data Analysis. The Statistical Design and Analysis of Gene Expression Data. Laboratory Automation for Microarray Experimentation. How to Evaluate a Microarray Scanner. The Role of Substrates in Microarray Experimentation and How to Choose the Correct Coating for Microarraying. Towards Highly Efficient Automated Hybridizations. Characterization of Microarray Hybridization Stoichiometry. Data Normalization Selection. Methods for Assessing Microarray Performance. Comparison of Different Normalization Methods for Applied Biosystems Expression Array System. A Systematic Comparison of Gene Expression Measurements Across Different Hybridization-Based Technologies. DNA Methylation Arrays: Methods and Analysis. Electronic Microarrays: Progress toward DNA Diagnostics. New Applications for Microarrays. Development of an Oligonucleotide Microarray for Mutational Analysis Using Single or Multiple Sample Hybridization. Development of an Integrated Molecular Diagnostic Test to Identify Respiratory Viruses. Microarrays in Neuroscience. Optimization of Protein Array Fabrication for Establishing High Throughput Ultra-Sensitive Microarray Assays for Cancer Research. Index.

 

O autorze

Gary Hardiman