Micro and Nano Technologies in Bioanalysis: Methods and Protocols

ISBN-13: 9781617794872 / Angielski / Miękka / 2011 / 668 str.

James W. Lee; Robert S. Foote

Opis

In recent years, large-scale advances in technology have led to greater understanding of the world at the biomolecular level. In this book, expert researchers from across the globe explore the technology which makes this analysis possible.

Recenzje

From the reviews: "This book provides a comprehensive overview of different modern methods with acknowledged practical interest. ... this book can be considered as unique and highly valuable. ... This book can be recommended for scientists, technicians and students in the fields of molecular and cell biology, biochemistry, microbiology, genetics, medical research and analytical chemistry. ... This book is an excellent source for interested users to learn about recent developments of emerging microtechnologies and nanotechnologies for bioanalysis." (Rosario Pereiro, Analytical and Bioanalytical Chemistry, November, 2009) "This book describes specific micro and nano techniques used in analyzing functions in both individual cells and animal models. It includes details and tips for using these methods. ... Written for scientists at many different levels, the book includes specific step-by-step instructions in each chapter that would be useful for senior scientists as well as bench technical assistants. ... will be a valuable resource for biomedical scientists." (Rebecca T. Horvat, Doody's Review Service, February, 2010)

Spis treści

Table of Contents Preface Contributors Part I. Applications of microfluidics and nanopores in separation, detection, manipulation, and analysis of biomolecules 1. HPLC-Chip/MS technology in proteomic profiling Martin Vollmer and Tom van de Goor 2. Nanofluidic channels fabrication and manipulation of DNA molecules Kai-Ge Wang, Hanben Niu 3. A single-molecule barcoding system using nanoslits for DNA analysis--Nanocoding Kyubong Jo, Timothy M. Schramm, David C. Schwartz 4. Microfluidic Devices with Photodefinable Pseudo-valves for Protein Separation Z. Hugh Fan 5. Microfluidic chip designed for measuring biomolecules through microbead-based quantum dot fluorescence assay Kwang-Seok Yun, Dohoon Lee, Hak-Sung Kim and Euisik Yoon 6. DNA Focusing Using Microfabricated Electrode Arrays Faisal A. Shaikh and Victor M. Ugaz 7. Solid-state Nanopore for Detecting Individual Biopolymers Jiali Li and Jene A. Golovchenko 8. Inserting and manipulating DNA in a nanopore with optical tweezers U. F. Keyser, J. van der Does, C. Dekker, N. H. Dekker 9. Forming an a-hemolysin nanopore for single molecule analysis Nahid N. Jetha, Matthew Wiggin, and Andre Marziali 10. Nanopore Force Spectroscopy on DNA Duplexes Nahid N. Jetha, Matthew Wiggin, and Andre Marziali Part II. Technologies of physical science and chemistry in detection and analysis of biomolecules 11. Quantitative chemical analysis of single cells Michael L. Heien and Andrew G. Ewing 12. Trapping and Detection of Single Molecules in Water M. Willander, K. Risveden, B. Danielsson and O. Nur 13. ZnO Nanorods as an Intracellular Sensor for pH Measurements M. Willander and Safaa Al-Hilli 14. Analysis of Biomolecules Using Surface Plasmons M. Willander and Safaa Al-Hilli 15. Use of residual dipolar couplings in structural analysis of protein-ligand complexes by solution NMR spectroscopy Nitin U. Jain 16. Raman-assisted X-ray crystallography for the analysis of biomolecules Dominique Bourgeois, Gergely Katona, Eve de Rosny and Philippe Carpentier 17. Methods and Software for Diffuse X-Ray Scattering from Protein Crystals Michael E. Wall 18. Deuterium Labeling for Neutron Structure-Function-Dynamics Analysis Flora Meilleur, Kevin L. Weiss, and Dean A.A. Myles 19. Small-angle Neutron Scattering for Molecular Biology: Basics and Instrumentation William T. Heller, Kenneth C. Littrell 20. Small-Angle Scattering and Neutron Contrast Variation for Studying Bio-molecular Complexes Andrew E. Whitten and Jill Trewhella 21. Protein sequencing with tandem mass spectrometry Assem G. Ziady and Michael Kinter 22. Metabolic Analysis Vladimir V. Tolstikov Part III. Applications of quantum dots and molecular fluorescence in detection, tracking and imaging of biomolecules 23. Multicolor detection of combed DNA molecules using quantum-dots Christophe Escudé, Bénédicte Géron-Landre, Aurélien Crut, and Pierre Desbiolles 24. Quantum Dot Molecular Beacons for DNA Detection Nathaniel C. Cady 25. Quantum dot hybrid gel blotting: a technique for identifying quantum dot-protein/ protein-protein interactions Tania Q. Vu and Hong Yan Liu 26. In vivo imaging of quantum dots Isabelle Texier, Véronique Josserand 27. Semiconductor Fluorescent Quantum dots: Efficient biolabels in cancer diagnostics Patricia M. A. Farias, Beate S. Santos, Adriana Fontes 28. The Monitoring and Affinity

Blurb

In recent years, large-scale advances in technology have led to greater understanding of the world on a much tinier scale: the biomolecular level. In Micro and Nano Technologies in Bioanalysis: Methods and Protocols, expert researchers from across the globe explore the technology which makes this analysis possible, investigating the worlds of microfluidics and nanotechnologies, and examining physical science techniques for the separation, detection, manipulation, and analysis of biomolecules. This volume contains innovative protocols on the application of microfluidics and the utilization of physical science-related technologies that will prove to be invaluable in the field of molecular biology. Chapters contain cutting edge applications of emerging nanotechnologies, including quantum dots and molecular fluorescence for the imaging and tracking of biomolecules. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and groundbreaking, Micro and Nano Technologies in Bioanalysis: Methods and Protocols is a necessary tool for cellular biologists, biochemists, microbiologists, geneticists and medical researchers alike.