Methods in Protein Biochemistry

ISBN-13: 9783110481501 / Angielski / Miękka / 2016 / 378 str.

Harald Tschesche

Opis

In this book, top researchers in the field of protein biochemistry describe modern methods to address the challenges of protein purification, folding and degradation. The significance of peptide purity for fibril formation, the use of target oriented peptide arrays, the design and application of protein epitope mimetics, and the structural resolving of the misfolding of mutant proteins enlarge are addressed.

Spis treści

Membrane protein folding in detergents, Deber Three phase partitioning (TPP), Dennison Protein import into the intermembrane space of mitochondria, Herrmann A novel PVDF membrane for on-membrane identification of gel-resolved proteins by MALDI-MS, Hirano Molecular mechanisms of chaperone action, Hoehfeld Proteomics of Duchenne muscle dystrophy, Kamp Analysis of protein complexes using chemical cross-linking and mass spectrometry, Leitner Determining the small molecule binding site of a protein by Capture Compound Mass Spectrometry (CCMS), Lenz Pathobiology of antitrypsin defiency and the serpinopathies, Lomas Wide-angle x-ray solution scattering method (WAXS), Makowski Bioaffinity-mass spectrometry: Molecular approaches and applications protein biochemistry, Przybylski Laser ablation electrospray ionization mass spectrometry (LAESI), Razunguzwa Design and applications of protein epitope mimetics, Robinson Probing protein dynamics in vivo: bacterial acyl carrier protein as a case study, Volkmann Target oriented peptide-arrays in a palliative approach to mucoviszidose, Volkmer Correlated single-crystal spectroscopy and x-ray crystallography, Orville Chemical modification of proteins in the living cell, Correa Jr. Relevance of peptide purity for the reproducibility of fibril formation, Glockshuber Proteome analysis of humen BAL by two-dimensional difference in gel-Electrophoresis (D-DIGE): COPD patients vs control subjects, Mileo, Beranova-Giorgianni, Giorgianni Dynamics of changes in conformation and folding processes in peptides and proteins, Kiefhaber, Reiner Glykoprotein folding within the endoplasmic reticulum, Parodi

O autorze

Harald Tschesche, University of Bielefeld, Germany.