ISBN-13: 9783110252330 / Angielski / Twarda / 2011 / 378 str.
ISBN-13: 9783110252330 / Angielski / Twarda / 2011 / 378 str.
This book presents a survey of recent developments in protein biochemistry. Top researchers in the field of protein biochemistry describe modern methods to address the challenges of protein purification by three-phase partitioning, and their folding and degradation by the functions of chaperones. The significance of peptide purity for fibril formation is addressed as well as the use of target oriented peptide arrays in palliative approaches in mucoviszidose. The design and application of protein epitope mimetics just as the structural resolving of the misfolding of various mutant proteins in serpinopathies enlarge our tools in resolving pathophysiological imbalances.
This book presents a survey of recent developments in protein biochemistry. As new analytical methods and techniques become available to the protein chemist for isolating, analyzing, and characterizing novel proteins, new insights into basic and applied biochemistry are made possible. Top researchers in the field of protein biochemistry describe modern methods to address the challenges of folding and misfolding, membrane proteins, fibril formation, molecular chaperones, protein interactions, evolution and design with numerous examples. Methods covered include laser ablation electrospray ionization mass spectrometry, multiparametric fluorescence microscopy imaging, bioaffinity-mass spectrometry, DNA reporters and biomarkers.