Methods in Non-Aqueous Enzymology

ISBN-13: 9783764361099 / Angielski / Miękka / 2000 / 218 str.

Munishwar Nath Gupta

Opis

Extending the range of enzymatic catalysis by using non-aqueous media considerably enlarges the range of enzyme applications and became a powerful approach in biochemistry and biotechnology. This book discusses several specific applications in nearly anhydrous media, aqueous-organic cosolvent mixtures and reverse micelles, including use of enzymes in fat and oil processing, peptide synthesis, sugar transformations and preparation of chiral compounds. A particularly promising approach of using reverse micelles for modifying proteins is also described. Almost each chapter covers background information, easy-to-follow protocols and illustrations of the specific applications.
Put together, the book is an important guideline for researchers and developers in biotechnological and pharmaceutical industries as well as academic sectors.

Spis treści

1. Non-Aqueous Enzymology: Issues and Perspectives.- 2. Importance of Water Activity for Enzyme Catalysis in Non-Aqueous Organic Systems.- 3. Engineering of Enzymes via Immobilization and Post-Immobilization Techniques: Preparation of Enzyme Derivatives with Improved Stability in Organic Media.- 4 Immobilization of Lipases for Use in Non-Aqueous Reaction Systems.- 5. Applications of Enzymes and Membrane Technology in Fat and Oil Processing.- 6. Strategies for Improving the Lipase-Catalyzed Preparation of Chiral Compounds.- 7. Peptide Synthesis in Non-Aqueous Media.- 8. Enzyme Selectivity in Organic Media.- 9. Sugar Transformations Using Enzymes in Non-Aqueous Media.- 10. Reversed Micelles as Microreactors: N-Terminal Acylation of RNase A and its Characterization.- 11. Analysis in Non-Aqueous Milieu Using Thermistors.- 12. Importance of the Medium forin vitroandin vivoProtein Folding Mechanisms: Biomedical Implications.