Methods in Membrane Lipids

ISBN-13: 9781617377037 / Angielski / Miękka / 2010 / 621 str.

Alex Dopico

Opis

Methods in Membrane Lipids presents a compendium of methodologies for the study of membrane lipids, varying from traditional lab bench experimentation to computer simulation and theoretical models. This volume provides a comprehensive set of techniques for studying membrane lipids, and their interactions with other membrane lipids, with membrane proteins, and with exogenous agents such as anesthetics. The progression of chapters increases in complexity, initially presenting introductory concepts and methods for the study of simple structures and interactions, then moving to the study of more complex structures and interactions, including: behaviors of lipids in water systems and phases; membrane lipid movements and diffusion; properties of lipids in mono and bilayers; complex lipid domains; and membrane-lipid interaction with proteins and drugs. Each chapter is written by an internationally recognized expert in the field.

Recenzje

From the reviews:

"A compilation of protocols that addresses the ... analysis of membrane lipid structure to polymorphism, lipid domains and (macro-) molecular interactions. It is a timely addition to the series on a topic that begs attention. It is a valuable reference material for researchers in lipidology and practitioners whose work involves understanding membrane structure and function. ... a valuable compilation of biophysical methodologies employed in the study of membrane lipids that will no doubt be useful to anyone with an interest in membrane structure and function." (Dr Seetharaman Vaidyanathan, Molecular Biotechnology, March, 2008)

"The book deals with the membrane models and biophysical approaches used to study them. It is a compendium of various experimental and theoretical methods for investigating lipids, lipid aggregates, and, to some extent, membrane proteins. ... The book is not only a collection of methods, but a textbook that can be recommended for scientists and students with a deeper interest in lipid biochemistry and biophysics. ... it can be highly recommended." (Thomas Kolter, ChemBioChem, Issue 9, 2008)

Spis treści

Methods in Membrane Lipids: Table of Contents Introductory Chapters: 1 A glance at the structural and functional diversity of membrane lipids Alejandro M. Dopico and Gabor J. Tigyi 2 Membrane lipid polymorphism: relationship to bilayer properties and protein function Richard Epand Lipid distribution, membrane lipid structure, and lipid-lipid interactions: 3 Acrylodan-labeled intestinal fatty acid binding protein to measure concentrations of unbound fatty acids Jeffrey R. Simard, Frits Kamp, and James A. Hamilton 4 Measuring molecular order and orientation using Coherent-anti Stokes Raman scattering microscopy Hilde A. Rinia, George W.H. Wurpel, and Michiel Müller 5 Preparation of oriented, fully hydrated lipid samples for structure determination using X-ray scattering Stephanie Tristram-Nagle 6 Nuclear Magnetic Resonance investigation of oriented lipid membranes Olivier Soubias and Klaus Gawrisch 7 Molecular dynamics simulations as a complement to Nuclear Magnetic Resonance and X-ray diffraction measurements Scott Feller 8 Use of inverse theory algorithms in the analysis of biomembrane Nuclear Magnetic Resonance data Edward Sternin 9 Statistical thermodynamics via computer simulation to characterize phospholipid interactions in membranes Mihaly Mezei and Pál Jedlovszky 10 Fluorometric assay for detection of sterol oxidation in liposomal membranes Parkson L-G. Chong and Michelle Olsher 11 Fluorescence detection of signs of sterol superlattice formation in lipid membranes Parkson L-G. Chong, Berenice Venegas, and Michelle Olsher Characterization of lipid phases: 12 Differential scanning calorimetry in the study of lipid phase transitions in model and biological membranes: practical considerations Ruthven N. A. H. Lewis, David A. Mannock, and Ronald N. McElhaney 13 Pressure-perturbationcalorimetry Heiko Heerklotz 14 Fourier transform infrared spectroscopy in the study of lipid phase transitions in model and biological membranes: practical considerations Ruthven N. A. H.Lewis and Ronald McElhaney 15 Optical dynamometry to study phase transitions in lipid membranes Rumiana Dimova and Bernard Pouligny Lipid movements and diffusion: 16 Fluorescence assays for measuring fatty acid binding and transport through membranes Kellen Brunaldi, Jeffrey R. Simard, Frits Kamp, Charu Rewal, Tanong Asawakarn, Paul O'Shea, and James A. Hamilton 17 Measurement of lateral diffusion rates in membranes by Pulsed Magnetic Field Gradient, Magic Angle-Spinning Proton Nuclear Magnetic Resonance Klaus Gawrisch and Holly Gaede 18 Using Fluorescence Recovery After Photobleaching to measure diffusion in membranes Conrad W. Mullineaux and Helmut Kirchhoff 19 Single molecule fluorescence microscopy to determine phospholipid lateral diffusion Michael J. Murcia, Sumit Garg, and Christoph Naumann 20 Modeling two- and three-dimensional diffusion Michael Saxton 21 Measurement of water and solute permeability by stopped-flow fluorimetry John C. Mathai and Mark L. Zeidel Pressure between lipids, mono- and bilayer lipid curvature and stress: 22 Fluorescence microscopy to study pressure between lipids in giant unilamelar vesicles Anna Celli, Claudia Y.C. Lee, and Enrico Gratton 23 X-ray scattering and solid-state deuterium Nuclear Magnetic Resonance probes of structural fluctuations in lipid membranes Horia I. Petrache and Michael F. Brown 24 Determination of lipid spontaneous curvature from X-ray examinations of inverted hexagonal phases Michael Kozlov 25 Shape analysis of giant vesicles with fluid phase coexistence by laser scanning microscopy to determine curvature, bending elasticity and line tension Samuel T. Hess, M