Metastasis Research Protocols

ISBN-13: 9781617370991 / Angielski / Miękka / 2010 / 310 str.

Susan A. Brooks; Udo Schumacher

Opis

Metastasis Research Protocols describes in detail all the methods needed to investigate why and how metastasis occurs. Volume I presents a comprehensive collection of established and leading-edge techniques for analyzing the expression of key molecules and for examining their production at the genetic level. Volume I focuses on the analysis and mapping of molecules produced by cells and tissues, and on the molecular biology underlying their expression. The traditional methods range from the histopathological and the immunocytochemical to SDS-PAGE, Western blotting, and enzyme zymography. Newer and more specialized techniques for analyzing the genetic aspects of metastasis include in situ hybridization to localize mRNAs, FISH, CGH, methylation analysis of CpG islands, RT-PCR, differential display. Volume II presents a comprehensive collection of established and leading-edge techniques for examining metastatic behavior in vitro and whole animal models. Methods are provided for the separation of cell lines with high and low metastatic potential, along with assay systems to test defined aspects of the metastatic cascade.These systems include cell migration assays, assays for matrix degradation enzymes, basement membrane degradation assays, adhesion assays, and assays of angiogenesis. The detailed descriptions of animal models for metastasis cover the use of immunodeficient animals, syngenic and transgenic models, and orthotopic models of metastasis.

Recenzje

This book is a valuable addition to the literature as it brings together key people in the field of cancer metastasis to provide practical step-by-step protocols. . . This book is written in such as way that it would be useful to beginning students and to anyone curious about the methods that are available to study the key steps in cancer metastasis. This book would also be useful to seasoned researchers as it brings together a number of different methodologies in one easy-to-read book. . . This is a very practical book covering areas of research in cancer invasion and metastasis. The greatest strength of the book is in collecting the different protocols that are useful in studying cellular behavior in one easy-to-read book.."-Weighted Numerical Score: 91 - 4 Stars!-Doody's Health Sciences Book Review Journal

"These volumes should be of interest for all medical and academic libraries where cancer research is performed." - E-Streams

Spis treści

Part I. Selection/Segregation of Cell Populations for In vitro and In Vivo Assays of Metastatic Behavior Cell Separations by Flow Cytometry Derek Davies Immunomagnetic Cell Separation Catherine Clarke and Susan Davies Genetic Modification of Cell Lines to Enhance Their Metastatic Capability Daniel McWilliams and Hilary Collins Part II. In Vitro Assays of Metastatic Behavior Cell Aggregation Assays, Tom Boterberg Marc E. Bracke, Erik A. Bruyneel, and Marc M. Mareel Cell Migration and the Boyden Chamber Nicholas S. Brown and Roy Bicknell Quantification of Cell Motility: Gold Colloidal Phagokinetic Track Assay and Wound Healing Assay Yasufumi Niinaka, Arayo Haga, and Avraham Raz In Vitro Invasion Assay Using Matrigel® Debbie M. S. Hall and Susan A. Brooks Membrane Invastion Culture System Mary J. C. Hendrix, Elisabeth A. Seftor, and Richard E. B. Seftor Collagen Invasion Assay Marc E. Bracke, Tom Boterberg, Erik A. Bruyneel, and Marc M. Mareel Chick Heart Invasion Assay Marc E. Bracke, Tom Boterberg, and Marc M. Mareel Adhesion of Tumor Cells to Matrices and Endothelium G. Ed Rainger Assessment of Angiogenic Factors: The Chick Chorioallantoic Membrane Assay Adam Jones, Chisato Fujiyama, Stephen Hague, and Roy Bicknell Methods to Evaluate the Formation and Stabilization of Blood Vessels and Their Role in Tumor Growth and Metastasis Nerbil Kilic and Süleyman Ergün Galectin-3 Binding and Metastasis Pratima Nangia-Makker, Yuichiro Honjo, and Avraham Raz Part III. Animal Models of Metastasis Basic Principles for the Study of Metastasis Using Animal Models Suzanne A. Eccles The Chick Embryo Metastasis Model Eric Petitclerc and Peter C. Brooks Gene Transfection for Metastasis ResearchUsing Animal Models Ruth J. Muschel and Jim Hua Theoretical Considerations in Using Animal Models of Metastastis and Brief Methodology for In Vivo Colorectal Cancer Models in SCID and Nude Mice Sue A. Watson and Teresa M. Morris Xenograft Models in Immunodeficient Animals: I. Nude Mice: Spontaneous and Experimental Metastatis Models Janet E. Price Xenograft Models in Immunodeficient Animals: II. Use of SCID Mice in Metastasis Research: Breast and Colon Cancer Models of Metastasis Udo Schumacher and Susan A. Brooks Syngeneic Murine Metastasis Model: B16 Melanoma Raffaella Giavazzi and Angela Garofalo Transgenic Animal Models Chantale Guy and Gizela Cordosa Clinically Accurate Orthotopic Mouse Models of Cancer Robert M. Hoffman Dissection of Tumor and Host Cells from Metastasized Organs for Testing Gene Expression Directly Ex Vivo Marian Rocha, Volker Schirrmacher, and Victor Umansky Green Fluorescent Protein for Metastastis Research Robert M. Hoffman

Blurb

Although ninety percent of fatal cancer cases involve the spread of a primary tumor, the formation of metastases is still a poorly understood, complex process and a significant problem in the treatment of cancer patients. In Metastasis Research Protocols, leading international investigators describe in detail the key methods needed to investigate why and how metastasis occurs. Volume II of this two-volume set, Analysis of Cell Behavior In Vitro and In Vivo, presents a comprehensive collection of established and leading-edge techniques for examining metastatic behavior in vitro and in whole animal models. Methods are provided for the separation of cell lines with high and low metastatic potential, along with assay systems to test defined aspects of the metastatic cascade. These systems include cell migration assays, assays for matrix degradation enzymes, basement membrane degradation assays, adhesion assays, and assays of angiogenesis. The detailed descriptions of animal models for metastasis cover the use of immunodeficient animals, syngenic and transgenic models, and orthotopic models of metastasis. The first volume of this set, Analysis of Cells and Tissues, focuses on the analysis and mapping of molecules produced by cells and tissues, and on the molecular biology underlying their expression.
Comprehensive and authoritative, the two volumes of Metastasis Research Protocols constitute a gold-standard collection of readily reproducible methods for understanding the metastatic cascade-first at the cellular and molecular levels, then at the level of the whole organism-responsible for the spread of cancer and for developing novel strategies to combat its spread.