Mass Spectrometry for Metabolomics

ISBN-13: 9781071627013 / Angielski / Miękka / 2023

Opis

This detailed volume provides a comprehensive overview of state-of-the-art metabolomics methods based on mass spectrometry (MS), and their application in food, nutrition, and biomedical research. The chapters assembled herein cover hot topics related to sample preparation, chromatographic and electrophoretic separation, MS-based analysis, as well as data processing and analysis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Mass Spectrometry for Metabolomics serves as a timely guide for chemists, biochemists, biologists, nutritionists, clinicians, and other experts working in the growing and exciting field of metabolomics.

Spis treści

1. Comprehensive and High-Throughput Method for Quantitative Fingerprinting of Phenolic Compounds

            Raúl González-Domínguez, Ana Sayago, María Santos-Martín, and Ángeles Fernández-Recamales

 

2. Determination of Volatile Metabolites in Vinegars by Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry (SPME-GC-MS)

            Remedios Castro and Enrique Durán-Guerrero

 

3. Determination of Volatile Metabolites in Vinegars by Stir Bar Sorptive Extraction-Gas Chromatography-Mass Spectrometry (SBSE-GC-MS)

            Enrique Durán-Guerrero and Remedios Castro

 

4. Discovery of Food Intake Biomarkers Using Metabolomics

            Leticia Lacalle-Bergeron, David Izquierdo-Sandoval, Juan V. Sancho, and Tania Portolés

 

5. Metabolomic Characterization of the Antiproliferative Activity of Bioactive Compounds from Fruit By-Products Against Colon Cancer Cells

            Gerardo Alvarez-Rivera, Alberto Valdés, and Alejandro Cifuentes

 

6. Untargeted Metabolomics by Liquid Chromatography-Mass Spectrometry in Biomedical Research

            Caridad Díaz and Carmen González-Olmedo

 

7. Untargeted Metabolomics for Disease-Specific Signatures

            Constantina Chalikiopoulou, Jose Carlos Gomez Tamayo, and Theodora Katsila

 

8. Metabolomics Analysis of Blood, Urine, and Saliva Samples Based on Capillary Electrophoresis-Mass Spectrometry

            Masahiro Sugimoto and Yumi Aizawa

 

9. Capillary Electrophoresis-Mass Spectrometry for the Direct Analysis of Metabolites in Highly Saline Samples Using In-Capillary Preconcentration

            Marlien van Mever and Rawi Ramautar

 

10. Chemical Derivatization to Enhance Capillary Electrophoresis Mass Spectrometric Analysis of Acidic Metabolites in Mammalian Cells

            Marlien van Mever and Rawi Ramautar

 

11. Untargeted Metabolomics Based on Liquid Chromatography-Mass Spectrometry for the Analysis of Plasma and Erythrocyte Samples in Childhood Obesity

            Álvaro González-Domínguez, Marina Armeni, Otto Savolainen, Alfonso María Lechuga-Sancho, Rikard Landberg, and Raúl González-Domínguez

 

12. Characterization of the Plasmatic and Erythroid Multi-Elemental Biodistribution in Childhood Obesity Using a High-Throughput Method for Size-Fractionation of Metal Species

            Álvaro González-Domínguez, María Millán-Martínez, Daniel Sánchez-Rodas, Alfonso María Lechuga-Sancho, and Raúl González-Domínguez

 

13. Analysis of Brain Metabolites Using Two Complementary Ultra-High Performance Liquid Chromatography Mass Spectrometry Methods

            Alexa M. Jauregui, Sofia E. Parellada, Emily Neag, and Sanjoy K. Bhattacharya

 

14. Analysis of Cholesterol from the Liver Using Gas Chromatography-Mass Spectrometry

            Meher A. Saleem, Betsy Benitez, Charles Yaros, Gabrielle Yamar, and Sanjoy K. Bhattacharya

 

15. Metabolomics Analysis of Aqueous Humor Based on Liquid Chromatography-Mass Spectrometry

            Karolina Pietrowska, Diana Anna Dmuchowska, Adam Kretowski, and Michal Ciborowski

 

16. Analyses and Localization of Serotonin and L-DOPA in Ocular Tissues by Imaging Mass Spectrometry

            Varun Krishnan, Sean Meehan, Colin Hayter, and Sanjoy K. Bhattacharya

 

17. Isobaric Incorporation of C13-Histidine for the Assessment of Remyelination

            Faith Christine Harvey, Anddre Osmar Valdivia, Colin Hayter, and Sanjoy K. Bhattacharya

 

18. Isolation and Lipidomic Screening of Human Milk Extracellular Vesicles

            Victoria Ramos-Garcia, Isabel Ten-Doménech, Abel Albiach-Delgado, Marta Gómez-Ferrer, Pilar Sepúlveda, Anna Parra-Llorca, Laura Campos-Berga, Alba Moreno-Giménez, Guillermo Quintás, and Julia Kuligowski

 

19. Mass Spectrometry-Based Untargeted Metabolomics and Lipidomics Platforms to Analyze Cell Culture Extracts

            Elias Iturrospe, Katyeny Manuela da Silva, Maria van de Lavoir, Rani Robeyns, Matthias Cuykx, Tamara Vanhaecke, Alexander L.N. van Nuijs, and Adrian Covaci

 

20. Data Processing and Analysis in Mass Spectrometry-Based Metabolomics

            Ángela Peralbo-Molina, Pol Solà-Santos, Alexandre Perera-Lluna, and Eduardo Chicano-Gálvez

 

21. Data Processing and Analysis in Liquid Chromatography-Mass Spectrometry-Based Targeted Metabolomics

            Masahiro Sugimoto, Yumi Aizawa, and Atsumi Tomita

 

22. Instrument-Agnostizing Methodology for Liquid Chromatography-Mass Spectrometry Systems

            Rosalía López-Ruíz, Sandra Martín-Torres, Ana M. Jiménez-Carvelo, Roberto Romero-González, and Luis Cuadros-Rodríguez