Mass Spectrometry-Based Lipidomics: Methods and Protocols

ISBN-13: 9781071614099 / Angielski / Twarda / 2021 / 314 str.

Fong-Fu Hsu

Spis treści

1. Mass Spectrometry-Based Lipidomics: An Overview

            Fong-Fu Hsu

 

2. High Confidence Shotgun Lipidomics Using Structurally Selective Ion Mobility-Mass Spectrometry

            Bailey S. Rose, Katrina L. Leaptrot, Rachel A. Harris, Stacy D. Sherrod, Jody C. May, and John A. McLean

 

3. Global Lipidomics Profiling by a High Resolution LC-MS Platform

            Thomas Züllig, Martin Trötzmüller, and Harald C. Köfeler

 

4. Comprehensive Structural Characterization of Lipids by Coupling Paternò–Büchi Reaction and Tandem Mass Spectrometry

            Qingyuan Hu, Yu Xia, and Xiaoxiao Ma

 

5. Chemical Derivatization-Aided High Resolution Mass Spectrometry for Shotgun Lipidome Analysis

            Vinzenz Hofferek, Huaqi Su, and Gavin E. Reid

 

6. Quantitative Analysis of Polyphosphoinositide, Bis(monoacylglycero)phosphate, and Phosphatidylglycerol Species by Shotgun Lipidomics after Methylation

            Meixia Pan, Chao Qin, and Xianlin Han

 

7. A Mass Spectrometry-Based Shotgun Lipidomics Using Charge-Switch Derivatization for Analysis of the Complex Long-Chain Fatty Acids

            Cheryl Frankfater and Fong-Fu Hsu

 

8. One-Pot Extractive Transesterification of Fatty Acids Followed by DMOX Derivatization for Location of Double Bonds Using GC-EI-MS

            Charles H. Hocart, Abdeljalil El Habti, and Gabriel O. James

 

9. Ceramide Analysis by Multiple Linked-Scan Mass Spectrometry Using a Tandem Quadrupole Instrument

            Fong-Fu Hsu

 

10. Comprehensive Mouse Skin Ceramide Analysis on a Solid-Phase and TLC Separation with High Resolution Mass Spectrometry Platform

            Meei-Hua Lin, Jeffrey H. Miner, and Fong-Fu Hsu

 

11. Mass Spectrometric Analysis of Meibomian Gland Lipids

            Jianzhong Chen

 

12. Quantification of Plasma Oxylipins Using Solid Phase Extraction and Reversed-Phase Liquid Chromatography-Triple Quadrupole Mass Spectrometry

            Guan-yuan Chen and Qibin Zhang

 

13. Targeted Lipidomics of Drosophila melanogaster During Development

            Esther Xue Yi Goh and Xue Li Guan

 

14. Shotgun Lipidomic Analysis of Leishmania Cells

            Kai Zhang and Fong-Fu Hsu

 

15. Characterization of the Uncommon Lipid Families in Corynebacterium glutamicum by Mass Spectrometry

            Raju V.V. Tatituri and Fong-Fu Hsu

 

16. Mass Spectrometric Analysis of Bioactive Sphingolipids in Fungi

            Ashutosh Singh and Maurizio Del Poeta

 

17. Analytical Methodologies for Lipidomics in Hemp Plant

            Andrea Cerrato, Anna Laura Capriotti, Carmela Maria Montone, Sara Elsa Aita, Giuseppe Cannazza, Cinzia Citti, Susy Piovesana, and Laganà Aldo

 

18. Shotgun Bacterial Lipid A Analysis Using Routine MALDI-TOF Mass Spectrometry

            Gérald Larrouy-Maumus

 

19. Imaging of Polar and Nonpolar Lipids Using Desorption Electrospray Ionization/Post-Photoionization Mass Spectrometry

            Chengyuan Liu, Fei Qi, and Yang Pan

 

20. Matrix-Assisted Laser Desorption/Ionization–Mass Spectrometry Imaging of Lipids in the Ischemic Rat Brain Section: A Practical Approach

            Hay-Yan J. Wang

Blurb

This detailed volume covers conventional MS-based “shotgun lipidomics” by which samples are introduced by infusion or loop injection, as well as LC-MS-based lipidomics, which are becoming increasingly important due to the ever-increasing demand for a complete and precise lipid analysis of the complex and diversified lipids in nature. The volume features protocols applying chemical reactions, the on-line photochemical reactions combined with various MS methods for comprehensive characterization of various lipid classes, and quantification of specific and rare lipids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Mass Spectrometry-Based Lipidomics: Methods and Protocols serves as an invaluable guide for biochemists and mass spectroscopists who are interested in lipid studies.