Mammalian and Avian Transgenesis - New Approaches

ISBN-13: 9783642066658 / Angielski / Miękka / 2010 / 281 str.

Shirley Pease; Carlos Lois

Opis

Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds. The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.

Spis treści

1 Generation of Transgenic Animals Using Lentiviral Vectors, Carlos Lois; 2 Intracytoplasmic Sperm Injection (ICSI) in the Mouse, Ming-Wen Li, K.C. Kent Lloyd; 3 Generation of Embryonic Stem (ES) Cell-Derived Embryos and Mice by Tetraploid-Embryo Complementation, Kevin Eggan, Rudolf Jaenisch; 4 Cloning The Laboratory Mouse by Nuclear Transfer, Kevin Eggan, Rudolf Jaenisch; 5 Large Insert Transgenesis, Shiaoching Gong, Nat Heintz; 6 Regional and Temporal Control of Genetic Manipulation in the Mouse, Mansuo L. Hayashi, Shigemi Hayashi; 7 High Resolution Gene Expression Analysis in Mice Using Genetically Inserted Reporter Genes, Niels C. Adams, Nicholas W. Gale; 8 Nuclear Transfer in the Cow, William A. Ritchie; 9 Production of Transgenic Pigs by DNA Microinjection, Robert M. Petters, Rebecca L. Krisher; 10 Production of Transgenic Birds Using Lentiviral Vectors, Benjamin B. Scott, Carlos Lois; 11 Ancillary Techniques, Shirley Pease

O autorze

The generation of transgenic mammals, mostly rodents, has become one of the most important approaches in biological and biomedical research, and is of utmost importance in the generation of genetically improved livestock. Edited by respected scholars at the California Institute of Technology, this book provides step-by-step protocols of this important technique. The manual covers not only mice and other rodents, it also describes methods successfully used in cattle, sheep and pigs, making it extremely useful for anyone working on mammalian transgenesis.