Mammalian Oocyte Regulation: Methods and Protocols

ISBN-13: 9781627031905 / Angielski / Twarda / 2012 / 311 str.

Hayden A. Homer

Opis

Mammalian oocytes occupy a critical nexus in reproduction as they not only contribute half the genetic makeup of the embryo but also provide virtually all of the cytoplasmic building blocks required for sustaining embryogenesis. The journey that transforms a primordial germ cell into a mature oocyte (or egg) capable of fertilisation and embryonic development is of unrivalled complexity. The end-product of this remarkable developmental process is the mature egg, now replete with macromolecules and organelles, and poised to support embryonic development if fertilisation should occur. Given the importance of the oocyte for embryonic development, it is of no surprise that the oocyte holds centre stage in fertility clinics and greatly influences the outcome of assisted reproductive treatments (ARTs). Mammalian Oocyte Regulation: Methods and Protocols provides a highly diverse compendium of detailed methodologies - primarily focusing on the murine model but also including chapters on human oocytes - for enabling researchers to interrogate every aspect of mammalian oocyte development including recombination, meiotic maturation, oocyte substrate uptake, chromosomal segregation and fertilisation. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Mammalian Oocyte Regulation: Methods and Protocols serves as an ideal guide to novice oocyte researchers, experts who wish to add another dimension to their established portfolio, as well as the wider research community. "

Spis treści

1. Studying Recombination in Mouse Oocytes

Xianfei Sun and Paula E. Cohen

 

2. Analysis of Recombinants in Female Mouse Meiosis

Esther de Boer, Maria Jasin, and Scott Keeney

 

3. Studying Meiosis Specific Cohesins in Mouse Embryonic Oocytes

Ji-hye Kim, Kei-ichiro Ishiguro, Nobuaki Kudo, and Yoshinori Watanabe

 

4. Immunohistochemical Approaches to the Study of Human Fetal Ovarian Development

Jing He, Andrew J. Childs, Jieqian Zhou, and Richard A. Anderson

 

5. Protein Kinase Assays for Measuring MPF and MAPK Activities in Mouse and Rat Oocytes and Early Embryos

Jacek Z. Kubiak

 

6. Time-Lapse Epi-Fluorescence Imaging of Expressed cRNA to Cyclin B1 for Studying Meiosis I in Mouse Oocytes

Janet E. Holt, Simon I.R. Lane, and Keith T. Jones

 

7. Using FRET to Study RanGTP Gradients in Live Mouse Oocytes

Julien Dumont and Marie-Hélène Verlhac

 

8. Making cRNA for Microinjection and Expression of Fluorescently-Tagged Proteins for Live-Cell Imaging in Oocytes

Mark Levasseur

 

9. RNAi-Based Methods for Gene Silencing in Mouse Oocytes

Paula Stein, Petr Svoboda, and Richard M. Schultz

 

10. Micro-Injection of Morpholino Oligonucleotides for Depleting Securin in Mouse Oocytes

Petros Marangos

 

11. Measuring Transport and Accumulation of Radiolabelled Substrates in Oocytes and Embryos

Jay M. Baltz, Hannah E. Corbett, and Samantha Richard

 

12. Immunofluorescence Staining of Spindles, Chromosomes and Kinetochores in Human Oocytes

            Solon Riris, Suzanne Cawood, Paul Serhal, and Hayden Homer

 

13. Studying the Roles of Aurora-C Kinase During Meiosis in Mouse Oocytes

Kuo-Tai Yang, Yi-Nan Lin, Shu-Kuei Li, and Tang K. Tang

 

14. Chromosome Spreads with Centromere Staining in Mouse Oocytes

Jean-Philippe Chambon, Khaled Hached, and Katja Wassmann

 

15. Preparation of Mammalian Oocytes for Transmission Electron Microscopy

            Lynne Anguish and Scott Coonrod

 

16. Measuring Ca2+ Oscillations in Mammalian Eggs

Karl Swann

 

17. Counting Chromosomes in Intact Eggs

Teresa Chiang and Michael A. Lampson

 

18. Free-Hand Bisection of Mouse Oocytes and Embryos

Zbigniew Polanski and Jacek Z. Kubiak

 

19. Microarray-CGH for the Assessment of Aneuploidy in Human Polar Bodies and Oocytes

Souraya Jaroudi and Dagan Wells

 

20. Nuclear Transfer in the Mouse Oocyte

Eiji Mizutani, Atsuo Ogura, and Teruhiko Wakayama

Petros Marangos

 

11. Measuring Transport and Accumulation of Radiolabelled Substrates in Oocytes and Embryos

Jay M. Baltz, Hannah E. Corbett, and Samantha Richard

 

12. Immunofluorescence Staining of Spindles, Chromosomes and Kinetochores in Human Oocytes

            Solon Riris, Suzanne Cawood, Paul Serhal, and Hayden Homer

 

13. Studying the Roles of Aurora-C Kinase During Meiosis in Mouse Oocytes

Kuo-Tai Yang, Yi-Nan Lin, Shu-Kuei Li, and Tang K. Tang

 

14. Chromosome Spreads with Centromere Staining in Mouse Oocytes

Jean-Philippe Chambon, Khaled Hached, and Katja Wassmann

 

15. Preparation of Mammalian Oocytes for Transmission Electron Microscopy

            Lynne Anguish and Scott Coonrod

 

16. Measuring Ca2+ Oscillations in Mammalian Eggs

Karl Swann

 

17. Counting Chromosomes in Intact Eggs

Teresa Chiang and Michael A. Lampson

 

18. Free-Hand Bisection of Mouse Oocytes and Embryos

Zbigniew Polanski and Jacek Z. Kubiak

 

19. Microarray-CGH for the Assessment of Aneuploidy in Human Polar Bodies and Oocytes

Souraya Jaroudi and Dagan Wells

 

20. Nuclear Transfer in the Mouse Oocyte

Eiji Mizutani, Atsuo Ogura, and Teruhiko Wakayama

Petros Marangos

 

11. Measuring Transport and Accumulation of Radiolabelled Substrates in Oocytes and Embryos

Jay M. Baltz, Hannah E. Corbett, and Samantha Richard

 

12. Immunofluorescence Staining of Spindles, Chromosomes and Kinetochores in Human Oocytes

            Solon Riris, Suzanne Cawood, Paul Serhal, and Hayden Homer

 

13. Studying the Roles of Aurora-C Kinase During Meiosis in Mouse Oocytes

Kuo-Tai Yang, Yi-Nan Lin, Shu-Kuei Li, and Tang K. Tang

 

14. Chromosome Spreads with Centromere Staining in Mouse Oocytes

Jean-Philippe Chambon, Khaled Hached, and Katja Wassmann

 

15. Preparation of Mammalian Oocytes for Transmission Electron Microscopy

            Lynne Anguish and Scott Coonrod

 

16. Measuring Ca2+ Oscillations in Mammalian Eggs

Karl Swann

 

17. Counting Chromosomes in Intact Eggs

Teresa Chiang and Michael A. Lampson

 

18. Free-Hand Bisection of Mouse Oocytes and Embryos

Zbigniew Polanski and Jacek Z. Kubiak

 

19. Microarray-CGH for the Assessment of Aneuploidy in Human Polar Bodies and Oocytes

Souraya Jaroudi and Dagan Wells

 

20. Nuclear Transfer in the Mouse Oocyte

Eiji Mizutani, Atsuo Ogura, and Teruhiko Wakayama

Petros Marangos

 

11. Measuring Transport and Accumulation of Radiolabelled Substrates in Oocytes and Embryos

Jay M. Baltz, Hannah E. Corbett, and Samantha Richard

 

12. Immunofluorescence Staining of Spindles, Chromosomes and Kinetochores in Human Oocytes

            Solon Riris, Suzanne Cawood, Paul Serhal, and Hayden Homer

 

13. Studying the Roles of Aurora-C Kinase During Meiosis in Mouse Oocytes

Kuo-Tai Yang, Yi-Nan Lin, Shu-Kuei Li, and Tang K. Tang

 

14. Chromosome Spreads with Centromere Staining in Mouse Oocytes

Jean-Philippe Chambon, Khaled Hached, and Katja Wassmann

 

15. Preparation of Mammalian Oocytes for Transmission Electron Microscopy

            Lynne Anguish and Scott Coonrod

 

16. Measuring Ca2+ Oscillations in Mammalian Eggs

Karl Swann

 

17. Counting Chromosomes in Intact Eggs

Teresa Chiang and Michael A. Lampson

 

18. Free-Hand Bisection of Mouse Oocytes and Embryos

Zbigniew Polanski and Jacek Z. Kubiak

 

19. Microarray-CGH for the Assessment of Aneuploidy in Human Polar Bodies and Oocytes

Souraya Jaroudi and Dagan Wells

 

20. Nuclear Transfer in the Mouse Oocyte

Eiji Mizutani, Atsuo Ogura, and Teruhiko Wakayama