Magnetomicrofluidic Circuits for Single-Bioparticle Transport

ISBN-13: 9789819917013 / Angielski

Roozbeh Abedini-Nassab

Spis treści

Abstract

1. Introduction

1.1 Motivation

1.2 Magnetic Techniques in Biology

1.2.1 In vivo Magnetic Techniques

1.2.2 In vitro Magnetic Techniques

1.3 Single-Cell Analysis Tools

1.3.1 Flow-Based Systems

1.3.2 Array-Based Systems

1.4 Circuit Theory

1.5 Magnetophoretic Circuits

2. Theory

2.1 Magnetic Materials

2.2 Magnetic Forces

2.3 Computational Methods

2.3.1 Analytical Solution

2.3.2 Finite-Element-Method-Based Analytical Solution

2.4 Particle Trajectory Analysis

3. Experimental Methods

3.1 Chip Microfabrication

3.2 Chip Surface Functionalization

3.3 Packing Methods

3.3.1 Chamber

3.3.2 Electrical Connections

3.4 Magnetic Field Apparatus

3.5 Control/Monitor System

3.6 Data and Image Analysis Methods

3.7 Cell Labeling

3.8 Magnetic Nanoparticle Fabrication

4. Particle Transport and Storage with Magnetophoretic Circuits

4.1 Magnetophoretic Circuits in Applied 2D Magnetic Field

4.1.1 Conductors

4.1.2 Diodes

4.1.3 Capacitors

4.2 Magnetophoretic Circuits in Applied 3D Magnetic Field

4.2.1 Conductors

4.2.2 Diodes

4.3 Magnetometamaterials

5. Particle Switching with Magnetic Transistors

5.1 Magnetophoretic Transistors in Applied 2D Magnetic Field

5.2 Magnetophoretic Transistors in Applied 3D Magnetic Field

6. Integrated Magnetomicrofluidics Circuits

6.1 Random Access Memory for Organizing Single Beads and Cells

6.2 Magnetomicrofluidics

6.2.1 Magnetomicrofluidics Circuit Theory

6.2.2 Magnetic Transfer in Magnetomicrofluidics Circuits

6.3 Improving the Microfluidics Fabrication Strategy

6.3.1 Surface Passivation inside Microfluidics Channels

6.3.2 Fabrication of Magnetomicrofluidics Chips based on Silicon and Glass Anodic Bonding

6.3.3 Interfacing the Microfluidics Chips to the World

6.3.4 Operation of the Magnetomicrofluidics Circuits

7. Biological Applications

7.1 Biocompatibility Tests

7.1.1 Nonadherent Cells

7.1.2 Adherent Cells

7.2 RNA-Seq Tests

7.3 Drug Screening Tests

8. Final Words and Future Directions 

 

 

O autorze

Roozbeh Abedini-Nassab is an Assistant Professor or Mechanical Engineering in Tarbiat Modares University. After earning his Bachelors and Master’s degrees in Electrical Engineering, Roozbeh Abedini-Nassab earned his Ph.D. in Mechanical Engineering from Duke University, and worked as a Postdoctoral Associate in the Biomedical Engineering Department at Cornell University. His research interest includes Biomechanics, BioMEMS, Biomedical Microdevices, Magnetic, Microfluidics, Lab-on-a-Chip systems, and Single-Cell analysis. He has published his research outcomes in high-impact journals, including Nature Communications, Lab on a Chip, Advanced Materials, Advanced Functional Materials, and so on. He has also filed US patent applications. Moreover, he has won several international awards for his presentations (e.g., best oral presentation award, best poster award, etc.), published articles (e.g., Editor’s Choice award), and research activities (e.g., Top Researcher Award).

Blurb

This book highlights the current state of the art in magnetophoretic circuits and their use in the emerging field of single-cell analysis. This interdisciplinary topic involves many fields of science including cellular biology, drug screening, cancer research, personalized medicine, microfabrication, biomedical microdevices, and lab-on-a-chip. This book not only provides the required fundamental knowledge and background needed in magnetics and the circuit theory but also describes the idea of magnetophoretic circuits as well as the cutting-edge developed technologies. It provides a sufficient background in i) the required theory in magnetics, ii) SCAs in general, iii) the circuit theory, iv) the developed idea of the magnetophoretic circuits, v) the fabrication process and magnetic cell labeling techniques, vi) the magnetophoretic-based SCA tools, and vii) the bio-applications. Methods for performing simulations as well as designing, fabricating, and running experiments are explained. Author of the book is one of the inventors of some of the ideas and the author/co-author of some of the related articles in high-impact journals. The book appeals to the readers interested in clinical applications as well as the ones interested in its technical aspects. It is beneficial for researchers interested in the field of single-cell analysis from various disciplines including biomedical engineering, mechanical engineering, electrical engineering, materials science, and cellular biology.