Macromolecular Crystallography Protocols, Volume 1: Preparation and Crystallization of Macromolecules

ISBN-13: 9781617374753 / Angielski / Miękka / 2010 / 280 str.

Sylvie Doublie

Opis

Macromolecular Crystallography Protocols, now in two volumes, examines major developments that have occurred since publication of the acclaimed first edition nearly a decade ago. Volume 1, Preparation and Crystallization of Macromolecules and Volume 2, Structure Determination, explore recent advances that have accelerated the pace of structural determination and made crystallography accessible to a broader range of investigators. Volume 1 is composed of detailed protocols for the preparation and optimization of crystals, including tips from the experts on the best methods for inducing proteins to adopt their crystalline form. Volume 2 complements the first volume by addressing laboratory techniques for crystal handling and structural characterization, as well as computational techniques for data collection, phasing, and refinement. The volume concludes with a detailed and insightful survey of available crystallographic software. These volumes will be an indispensable reference for obtaining macromolecular crystals and determining their three-dimensional structure.

Spis treści

TABLE OF CONTENTS VOLUME 1 HUMANA PRESS-CRYSTALLOGRAPHIC METHODS AND PROTOCOLS Sylvie Doublié, Editor CHAPTER TITLE AUTHOR(S) PAGE 1. A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual His6-MBP Affinity Tag Joseph E. Tropea, Scott Cherry, Sreedevi Nallamsetty, Christophe Bignon, and David S.Waugh 1-29 2. Cloning, production, and purification of proteins for a medium scale structural genomics project Sophie Quevillon-Cheruel, Bruno Collinet, Lionel Trésaugues, Philippe Minard, Gilles Henckes, Robert Aufrère, Karine Blondeau, Cong-Zhao Zhou, Dominique Liger, Nabila Bettache, Anne Poupon, Ilham Aboulfath, Nicolas Leulliot, Joel Janin, and Herman van Tilbeurgh 1-26 3. Baculoviral expression of an integral membrane protein for structural studies Dean R. Madden and Markus Safferling 1-33 4. Protein Engineering Sonia Longhi, François Ferron, and Marie-Pierre Egloff 1-48 5. Selenomethionyl protein expression Sylvie Doublié 1-21 6. How to Use Dynamic Light Scattering to Improve Your Chances of Growing Crystals of a Macromolecule Gloria Borgstahl 1-34 7. Screening and optimization methods for non-automated crystallization laboratories Terese Bergfors 1-32 8. Improving Marginal Crystals Charles W. Carter, Jr. and Madeleine Riès-Kautt 1-34 9. Optimisation Techniques for Automation and High Throughput Naomi E. Chayen 1-26 10. Three-dimensional crystallization of membrane proteins James Féthière 1-40 11. Crystallization of Protein-DNA complexes Thomas Hollis 1-20 12. Preparation and crystallization of RNA Barbara Golden 1-24 13. Crystallisation of RNA-protein complexes Eiji Obayashi, Chris Oubridge, Daniel Pomeranz Krummel, and Kiyoshi Nagai 1-27