Lung Cancer: Volume 1: Molecular Pathology Methods and Reviews

ISBN-13: 9780896039858 / Angielski / Twarda / 2002 / 594 str.

Robert Hopkins Miller; Barbara Driscoll; Barbara Driscoll

Opis

The Methods in Molecular Medicine series is intended as a resource for both novice and experienced investigators attempting to diversify their tech- logical base in research. Lung Cancer: Volume 1: Molecular Pathology Me- ods and Reviews presents an overview of the current status of assays employed to detect and characterize the multitude of pathologies that contribute to the development of this deadly disease. As with all volumes in the Methods in Molecular Medicine series, the reader should find that each methods-based chapter provides clear instructions for the performance of various protocols, supplemented by additional technical notes that provide valuable insight. These notes are designed to enable the reader to acquire the techniques described with a proficiency not easily achieved by re- ing standard method formats. No volume can exhaustively cover every aspect of biological research and there will be gaps in this endeavor that one or another research group will id- tify. Each section herein could readily be expanded into a book in its own right. However, I have sought to include a spectrum of techniques that should allow for the acquisition of key skills in each area covered.

Spis treści

Part I. Introduction Characteristic Genetic Alterations in Lung Cancer Ignacio I. Wistuba and Adi F. Gazdar Part II. Etiology and Classification of Lung Tumors Clinical and Biological Relevance of Recently Defined Categories of Pulmonary Neoplasia Edward Gabrielson Molecular Epidemiology of Human Cancer Risk: Gene-Environment Interactions and p53 Mutation Spectrum in Human Lung Cancer Kirsi Vähäkangas Neuroendocrine Phenotype of Small Cell Lung Cancer Judy M. Coulson, Marta Ocejo-Garcia, and Penella J. Woll AIDS-Associated Pulmonary Cancers Kushagra Katariya and Richard J. Thurer Part III. Molecular Abnormalities in Lung Cancer A. Detection of Alterations in the Cell Cycle. Abrogation of the RB-p16 Tumor Suppressor Pathway in Human Lung Cancer Joseph Geradts Sensitive Detection of Hypermethylated p16INK4a Alleles in Exfoliative Tissue Material Marcus Schuermann and Michael Kersting B. Detection of Alterations in Signal Transduction Pathways Role of Receptor Tyrosine Kinases in Lung Cancer Gautam Maulik, Takashi Kijima, and Ravi Salgia Relationship of EGFR Signal-Transduction Modulation by Tyrosine Kinase Inhibitors to Chemosensitivity and Programmed Cell Death in Lung Cancer Cell Lines Wendong Lei, Patrick P. Koty, and Mark L. Levitt Detection of the Transcripts and Proteins for the Transforming Growth Factor-b Isoforms and Receptors in Mouse Lung Tumorigenesis Using In Situ Hybridization and Immunohistochemistry in Paraffin-Embedded Tissue Sections Sonia B. Jakowlew and Jennifer Mariano Alterations in the Expression of Insulin-Like Growth Factors and Their Binding Proteins in Lung Cancer Carrie M. Coleman and Adda Grimberg Screening of Mutations in the ras Family of Oncogenes by Polymerase Chain Reaction-Based Ligase Chain Reaction AlfredoMartínez, Teresa A. Lehman, Rama Modali, and James L. Mulshine Assays for Raf-1 Kinase Phosphorylation and Activity in Human Small Cell Lung Cancer Cells Rajani K. Ravi and Barry D. Nelkin g-Glutamylcysteine Synthetase in Lung Cancer: Effect on Cell Viability Kristiina Järvinen, Ylermi Soini, and Vuokko L. Kinnula Localization of Cyclooxygenase-2 Protein Expression in Lung Cancer Specimens by Immunohistochemical Analysis Sarah A. Wardlaw and Thomas H. March Non-RI Protocols for L-myc Allelotyping and Deletion Mapping of Chromosome 1p in Primary Lung Cancers Keiko Hiyama, Ciro Mendoza, and Eiso Hiyama C. Detection of Altered Cell Surface Markers Intercellular Adhesion Molecules (ICAM-1, VCAM-1, and LFA-1) in Adenocarcinoma of Lung: Technical Aspects Armando E. Fraire, Bruce A. Woda, Louis Savas, and Zhong Jiang MUC1 Expression in Lung Cancer Eva Szabo Altered Surface Markers in Lung Cancer: Lack of Cell-Surface Fas/APO-1 Expression in Pulmonary Adenocarcinoma May Allow Escape from Immune Surveillance Yoshihiro Nambu and David G. Beer D. Detection of Alterations in the Apoptotic Pathway Prognostic Relevance of Angiogenic, Proliferative, and Apoptotic Factors in Lung Carcinomas: A Case Review Manfred Volm and Reet Koomägi Morphological Assessment of Apoptosis in Human Lung Cells Lori D. Dwyer-Nield, David Dinsdale, J. John Cohen, Margaret K. T. Squier, and Alvin M. Malkinson [D-Arg6, D-Trp7,9, NmePhe8]-Substance P (6-11) Activates JNK and Induces Apoptosis in Small Cell Lung Cancer Cells via an Oxidant-Dependent Mechanism Alison MacKinnon and Tariq Sethi Isolation of Cells Entering Different Programmed Cell Death Pathways Using a Discontinuous Percoll Gradient Patrick P. Koty, Haifan Zhang, Wendong Lei, and Mark L. Levitt E. Detection of Alterations in Lung

Blurb

Molecular-based laboratory methods can help significantly in the diagnosis and treatment of lung cancer, a leading cause of death worldwide. In Lung Cancer, Volume 1: Molecular Pathology Methods and Reviews, leading physicians, scientists, and noted researchers review novel methods for determining the etiology of a variety of lung cancers and present readily reproducible techniques for examining the associated multitude of genetic abnormalities. The methods described make it possible to detect these alterations at the cellular, DNA, and protein levels, to study the development of lung cancer in vitro and in vivo-either in situ or in the form of metastases-and to test targeted therapies with detailed model systems. An animal models section gives explicit instructions for setting up and testing these systems, which are rarely described in the literature. A companion volume, Lung Cancer, Volume 2: Diagnostic and Therapeutic Methods and Reviews, concentrates on diagnosing lung cancer through molecular analysis of clinical samples and providing a variety of novel therapies.
Comprehensive and informative, Lung Cancer, Volume 1: Molecular Pathology Methods and Reviews illuminates the etiology of lung cancer and provides reliable techniques for detecting the responsible genetic mutations at the cellular, DNA, and protein levels. Together, the two volumes constitute an unprecedented resource for those who wish to better understand how this disease is being diagnosed and treated and what novel techniques may be available to improve both processes.