Liquid Chromatography-Mass Spectrometry

ISBN-13: 9780824740825 / Angielski / Twarda / 2006 / 632 str.

Wilfried M. A. Niessen

Opis

Illustrates how LC-MS works, how to interpret results, and why it is useful across many disciplines Focuses on principles, technologies, applications, and analytical strategies in a single volume Dedicates more than two-thirds of the text to the impact of LC-MS on an ever-expanding list of applications Introduces customized strategies for the most important application areas of LC-MS Contains concise introductions and up-to-date references for further study in each chapter A constructive evaluation of the most significant developments in liquid chromatography-mass spectrometry (LC-MS) and its uses for quantitative bioanalysis and characterization for a diverse range of disciplines, Liquid Chromatography-Mass Spectrometry, Third Edition offers a well-rounded coverage of the latest technological developments and applications. As the technology itself has matured into a reliable analytical method over the last 15 years, the most exciting developments occur in LC-MS augments research into new applications. This edition places a stronger emphasis than previous editions on the impact of LC-MS methods, dedicating two-thirds of the text to small-molecule and biomolecular applications such as proteomics, pharmaceutical drug discovery and development, biochemistry, clinical analysis, environmental studies, and natural products research. Supported by the most relevant literature available, each chapter examines how the strategies, technologies, and recent advances-from sample pretreatment to data processing-in LC-MS helped to shape these disciplines. Featuring new chapters and extensive revisions throughout the book, Liquid Chromatography-Mass Spectrometry, Third Edition continues to provide scientists with a definitive guide and reference to the most important principles, strategies, and experimental precedents for applying LC-MS to their research.

Spis treści

INTRODUCTION
Liquid Chromatography and Sample Pretreatment
Mass Spectrometry
TECHNOLOGY
Strategies in LC–MS Interfacing
History of LC–MS Interfaces
Interfaces for Atmospheric-Pressure Ionization
Atmospheric-Pressure Ionization
APPLICATIONS: SMALL MOLECULES
LC–MS Analysis of Pesticides
Environmental Applications of LC–MS
LC–MS in Drug Discovery and Development
LC–MS in Drug Metabolism
Quantitative Bioanalysis Using LC–MS
Clinical Applications of LC–MS
LC–MS Analysis of Steroids
LC–MS in Food Safety Analysis
LC–MS Analysis of Plant Phenols
APPLICATIONS: BIOMOLECULES
LC–MS analysis of Proteins
LC–MS analysis of Peptides / Enabling Technology
LC–MS in Proteomics
LC–MS for Identification of Post-Translational Modifications
LC–MS Analysis of Oligosaccharides
LC–MS Analysis of Lipids and Phospholipids
LC–MS Analysis of Nucleic Acids INDEX

O autorze

Wilfried M.A. Niessen