Lipid Rafts: Methods and Protocols

ISBN-13: 9781071608166 / Angielski / Miękka / 2021 / 350 str.

Erhard Bieberich

Spis treści

Foreword…
Preface…
Table of Contents…
Contributing Authors…

1. Isolation and Analysis of Lipid Rafts from Neural Cells and Tissues
Sara Grassi, Paola Giussani, Laura Mauri, Simona Prioni, and Alessandro Prinetti

2. A Detergent-Free Method for Preparation of Lipid Rafts for the Shotgun Lipidomics Study
Chao Qin, Meixia Pan, and Xianlin Han

3. Biochemical Analysis of Lipid Rafts to Study Pathogenic Mechanisms of Neural Diseases
Ernesto R. Bongarzone and Maria Irene Givogri

4. Amyloid- β Interactions with Lipid Rafts in Biomimetic Systems: A Review of Laboratory Methods
Galya Staneva, Chiho Watanabe, Nicolas Puff, Vesela Yordanova, Michel Seigneuret, and Miglena Angelova

5. Extracellular Vesicles Containing Ceramide-Rich Platforms: “Mobile Raft” Isolation and Analysis
Ahmed Elsherbini, Haiyan Qin, Zhihui Zhu, Priyanka Tripathi, Guanghu Wang, Simone M. Crivelli, Stefanka D. Spassieva, and Erhard Bieberich

6. Isolation of Lipid Rafts (Detergent-Resistant Microdomains) and Comparison to Extracellular Vesicles (Exosomes)
Glyn Dawson

7. Methods to Characterize Synthesis and Degradation of Sphingomyelin at the Plasma Membrane and Its Impact on Lipid Raft Dynamics
Mariana Nikolova-Karakashian

8. Association of Glycolipids and Growth Factor Receptors in Lipid Rafts
Jing Wang and Robert K. Yu

9. Lipid Raft Isolation by Sucrose Gradient Centrifugation and Visualization of Raft-Located Proteins by Fluorescence Microscopy: The Use of Combined Techniques to Assess Fas/CD95 Location in Rafts during Apoptosis Triggering
Consuelo Gajate and Faustino Mollinedo

10. Elucidating the Role of Lipid Rafts on G Protein-Coupled Receptor Function in the Mouse Kidney: An In Vivo Approach
Laureano D. Asico, Selim Rozyyev, Annabelle M. Crusan, Pedro A. Jose, and Van Anthony M. Villar

11. Analysis of Lipids in Ceramide-Enriched Membrane Domains
Alexander Carpinteiro and Erich Gulbins

12. Chemotherapeutic Agents-Induced Ceramide Rich Platforms (CRPs) in Endothelial Cells and their Modulation
Prerna R. Nepali and Adriana Haimovitz-Friedman

13. Biophysical Analysis of Lipid Domains by Fluorescence Microscopy
Ana E. Ventura, Tânia C. B. Santos, Joaquim T. Marquês, Rodrigo F.M. de Almeida, and Liana C. Silva

14. Biophysical Analysis of Lipid Domains in Mammalian and Yeast Membranes by Fluorescence Spectroscopy
Carla Sousa, Filipa C. Santos, Andreia Bento-Oliveira, Beatriz Mestre, Liana C. Silva, and Rodrigo F. M. de Almeida

15. Characterization of Lipid Order and Domain Formation in Model Membranes Using Fluorescence Microscopy and Spectroscopy
Andrew Fuhrer and Amir M. Farnoud

16. Characterization of the Effect of Sphingolipid Accumulation on Membrane Compactness, Dipole Potential, and Mobility of Membrane Components
Gyula Batta, Tímea Hajdu, and Peter Nagy

17. Super-Resolution Microscopy of Sphingolipids
Jan Schlegel and Markus Sauer

18. Detection of Functionalized Sphingolipid Analogues in Detergent Resistant Membranes of the Immune Cells  
Julian Fink, Jürgen Seibel, and Elita Avota

19. Immunofluorescence Labeling of Lipid-Binding Proteins CERTs to Monitor Lipid Rafts Dynamics
Caterina Giovagnoni, Simone M. Crivelli, Mario Losen, and Pilar Martinez-Martinez

20. Cross-Link/Proximity Ligation Assay for Visualization of Lipid and Protein Complexes in Lipid Rafts
Priyanka Tripathi, Zhihui Zhu, Haiyan Qin, Ahmed Elsherbini, Emily A. Roush, Simone M. Crivelli, Stefanka D. Spassieva, and Erhard Bieberich

Subject Index List…

Blurb

This volume gives a comprehensive insight into established and novel methods to analyze the structure and function of lipid rafts. This book covers topics such as isolation of lipid rafts and their functional analysis using biochemical methods; visualization of lipid rafts and their interaction with proteins using fluorescence-related methods; preparation of giant lipid vesicles and fluorescence spectroscopy; FRET and FRAP; and using photo-activated cross-linking of a ceramide analog combined with proximity ligation assay. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Lipid Rafts: Methods and Protocols is a valuable resource for both novice and expert researchers interested in learning more about the function of lipid rafts in many areas of cell biology and medicine.