Lipid-Activated Nuclear Receptors: Methods and Protocols

ISBN-13: 9781493991297 / Angielski / Twarda / 2019 / 220 str.

Matthew C. Gage; Ines Pineda-Torra

Spis treści

1. Quantification of Oxysterol Nuclear Receptor Ligands by LC/MS/MS

            Lilia Magomedova and Carolyn L. Cummins

 

2. A Stable Luciferase Reporter System to Characterise LXR Regulation by Oxysterols and Novel Ligands

            Samantha A. Hutchinson and James L. Thorne

 

3. Isolation and Analysis of Human Monocytes and Adipose Tissue Macrophages

Jean-Baptiste Julla, Raphaelle Ballaire, Tina Ejlalmanesh, Jean-François Gautier, Nicolas Venteclef, and Fawaz Alzaid

 

4. Glucan Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Adipose Tissue Macrophages

            Emelie Barreby, André Sulen, and Myriam Aouadi

 

5. Isolation and Purification of Tissue Resident Macrophages for the Analysis of Nuclear Receptor Activity

            Laura Alonso-Herranz, Jesús Porcuna, and Mercedes Ricote

 

6. Bone Marrow-Derived Macrophage Immortalization of LXR Nuclear Receptor-Deficient Cells

            Ana Ramón-Vázquez, Juan Vladimir de la Rosa, Carlos Tabraue, and Antonio Castrillo

 

7. Dual Crosslinking Chromatin-Immunoprecipitation Protocol for Next Generation Sequencing (ChIPseq) in Macrophages

            David A. Rollins and Inez Rogatsky

 

8. Analysis of LXR Nuclear Receptor Cistrome through ChIP-seq Data Bioinformatics

            Juan Vladimir de la Rosa, Ana Ramón-Vázquez, Carlos Tabraue, and Antonio Castrillo

 

9. Quantifying Cellular Cholesterol Efflux

            Sabrina Robichaud and Mireille Ouimet

 

10. Methods for Assessing the Effects of LXR Agonists on Macrophage Bacterial Infection

            Estibaliz Glaría, Jonathan Matalonga, and Annabel F. Valledor

 

11. Measuring Apoptotic Cell Engulfment (Efferocytosis) Efficiency

            Matthew C. Gage

 

12. Methods to Study Monocyte and Macrophage Trafficking in Atherosclerosis Progression and Resolution

            Ada Weinstock and Edward A. Fisher

 

13. Preparation of Frozen Liver Tissues for Integrated Omics Analysis

            Ning Liang, Rongrong Fan, Saioa Goni, and Eckardt Treuter

 

14. Identification of Nuclear Receptor Targets By Chromatin Immunoprecipitation in Fatty Liver

            Natalia Becares and Inés Pineda-Torra

 

15. The LPS/D-Galactosamine-Induced Fulminant Hepatitis Model to Assess the Role of Ligand-Activated Nuclear Receptors on the NLRP3 Inflammasome Pathway In Vivo

            Yasmine Sebti, Lise Ferri, Mathilde Zecchin, Justine Beauchamp, Denis Mogilenko, Bart Staels, Hélène Duez, and Benoit Pourcet

 

16. Analyzing T-Cell Plasma Membrane Lipids by Flow Cytometry

            Kirsty E. Waddington, Inés Pineda-Torra, and Elizabeth C. Jury

Blurb

This book covers a wide range of state-of-the-art methodologies and detailed protocols currently used to study the actions that lipid-activated nuclear receptors and their co-regulators have in tissues and immune cell types considered classic metabolic “powerhouses”. This includes the liver, adipose tissue, and monocytes/macrophages present in these and other metabolic tissues. While the main focus is on the oxysterol receptor or Liver X Receptor (LXR), the majority of the methods described can be easily applied to multiple nuclear receptors, as well as to other tissues or cell types. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Lipid-Activated Nuclear Receptors: Methods and Protocols serves as an ideal guide for researchers pursuing the vital study of nuclear receptor biology and beyond.