Jasmonate Signaling: Methods and Protocols

ISBN-13: 9781627034135 / Angielski / Twarda / 2013 / 347 str.

Alain Goossens; Laurens Pauwels

Opis

It is now well established that jasmonates, originally identified as the major component of jasmine scent, play a universal role in the plant kingdom and are involved in the regulation of diverse aspects of plant biology, including growth, development, metabolism, and interaction with the environment. In Jasmonate Signaling: Methods and Protocols, experts in the field aim to unite powerful emerging omics platforms with a number of key reductionist approaches to form a comprehensive collection of tools and protocols. The detailed chapters in this book embrace physiological, environmental, molecular, omics, and bioinformatics approaches that allow dissecting jasmonate actions in the model species Arabidopsis thaliana or in other plants. Written in the highly successful Methods in Molecular Biology series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, along with tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Jasmonate Signaling: Methods and Protocols will empower interested researchers to dissect all steps of jasmonate signaling and the processes they modulate.

Spis treści

Part I: Physiology

 

1. Phenotyping Jasmonate Regulation of Senescence

            Martin A. Seltmann and Susanne Berger

 

2. Characterizing Jasmonate Regulation of Male Fertility in Arabidopsis

            Bryan Thines, Ajin Mandaokar, and John Browse

 

3. Phenotyping Jasmonate Regulation of Root Growth

            Fabian Kellermeier and Anna Amtmann

 

Part II: Environment

 

4. Bioassays for Assessing Jasmonate-Dependent Defenses Triggered by Pathogens, Herbivorous Insects, or Beneficial Rhizobacteria

            Saskia C.M. Van Wees, Johan A. Van Pelt, Peter A.H.M. Bakker, and Corné M.J. Pieterse

 

5. Elicitation of Jasmonate-Mediated Defense Responses by Mechanical Wounding and Insect Herbivory

            Marco Herde, Abraham J.K. Koo, and Gregg A. Howe

 

6. Pseudomonas syringae Infection Assays in Arabidopsis

            Jian Yao, John Withers, and Sheng Yang He

 

7. Jasmonate Signaling in the Field, Part I: Elicited Changes in Jasmonate Pools of Transgenic Nicotiana attenuata Populations

            Emmanuel Gaquerel, Michael Stitz, Mario Kallenbach, and Ian T. Baldwin

 

8. Jasmonate Signaling in the Field, Part II: Insect-Guided Characterization of Genetic Variations in Jasmonate-Dependent Defenses of Transgenic and Natural Nicotiana attenuata Populations

            Emmanuel Gaquerel, Michael Stitz, Mario Kallenbach, and Ian T. Baldwin

 

Part III: Molecules

 

9. Profiling of Jasmonic Acid-Related Metabolites and Hormones in Wounded Leaves

            Yusuke Jikumaru, Mitsunori Seo, Hideyuki Matsuura, and Yuji Kamiya

 

10.  A Non-Targeted Approach for Extended Liquid Chromatography-Mass Spectrometry Profiling of Free and Esterified Jasmonates After Wounding

            Gaëtan Glauser and Jean-Luc Wolfender

 

11. Cell-Specific Detection of Jasmonates by Means of an Immunocytological Approach

            Bettina Hause, Kati Mielke, and Susanne Forner

 

12. Jasmonic Acid-Amino Acid Conjugation Enzyme Assays

            Martha L. Rowe and Paul E. Staswick

 

13. Pull-Down Analysis of Interactions Among Jasmonic Acid Core Signaling Proteins

            Sandra Fonseca and Roberto Solano

 

14. Yeast Two-Hybrid Analysis of Jasmonate Signaling Proteins

            Amparo Cuéllar Pérez, Laurens Pauwels, Rebecca de Clercq, and Alain Goossens

 

15. Modified Bimolecular Fluorescence Complementation Assay to Study the Inhibition of Transcription Complex Formation by JAZ Proteins

            Tiancong Qi, Susheng Song, and Daoxin Xie

 

16. Agroinfiltration of Nicotiana benthamiana Leaves for Co-Localization of Regulatory Proteins Involved in Jasmonate Signaling

            Volkan Çevik and Kemal Kazan

 

17. Electrophoretic Mobility Shift Assay for the Analysis of Interactions of Jasmonic Acid-Responsive Transcription Factors with DNA

            Johan Memelink

 

18. Transient Expression Assays in Tobacco Protoplasts

            Robin Vanden Bossche, Brecht Demedts, Rudy Vanderhaeghen, and Alain Goossens

 

19. Functional Analysis of Jasmonic Acid-Responsive Secondary Metabolite Transporters

            Nobukazu Shitan, Akifumi Sugiyama, and Kazufumi Yazaki

 

20. Expression Analysis of Jasmonate-Responsive Lectins in Plants

            Nausicaä Lannoo and Els J.M. Van Damme

 

Part IV: Omics

 

21. Profiling the Jasmonic Acid Responses by Nuclear Magnetic Resonance-Based Metabolomics

            Hye Kyong Kim, Young Hae Choi, and Robert Verpoorte

 

22. Metabolite Profiling of Plant Tissues by Liquid Chromatography Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

            Jacob Pollier and Alain Goossens

 

23. cDNA-AFLP-Based Transcript Profiling for Genome-Wide Expression Analysis of Jasmonate-Treated Plants and Plant Cultures

            Janine Colling, Jacob Pollier, Nokwanda P. Makunga, and Alain Goossens

 

24. Analysis of RNA-Seq Data with TopHat and Cufflinks for Genome-Wide Expression Analysis of Jasmonate-Treated Plants and Plant Cultures

            Jacob Pollier, Stephane Rombauts, and Alain Goossens

 

25. Transcriptome Coexpression Analysis Using ATTED-II for Integrated Transcriptomics/Metabolomics Analysis

            Keiko Yonekura-Sakakibara and Kazuki Saito

 

26. A Guide to CORNET for the Construction of Coexpression and Protein-Protein Interaction Networks

            Stefanie De Bodt and Dirk Inzé