Ion Mobility-Mass Spectrometry: Methods and Protocols

ISBN-13: 9781071600320 / Angielski / Miękka / 2020 / 312 str.

Giuseppe Paglia; Giuseppe Astarita

Spis treści

1. Fundamentals of Ion Mobility-Mass Spectrometry for the Analysis of Biomolecules

            Caleb B. Morris, James C. Poland, Jody C. May, and John A. McLean

 

Part I: Metabolomics and Lipidomics

 

2. Utilizing Drift Tube Ion Mobility Spectrometry for the Evaluation of Metabolites and Xenobiotics

            Melanie T. Odenkirk and Erin S. Baker

 

3. Untargeted Differential Metabolomics Analysis Using Drift Tube Ion Mobility-Mass Spectrometry

            Rick Reisdorph, Cole Michel, Kevin Quinn, Katrina Doenges, and Nichole Reisdorph

 

4. Drift Tube Ion Mobility-Mass Spectrometry for Non-Targeted 'Omics

            Tim J. Causon, Ruwan T. Kurulugama, and Stephan Hann

 

5. The Use of DMS-MS for the Quantitative Analysis of Acylcarnitines

            Nicholas B. Vera, Michelle Clasquin, Stephen L. Coy, and Paul Vouros

 

6. Traveling Wave Ion Mobility-Mass Spectrometry to Enhance the Detection of Low Abundance Features in Untargeted Lipidomics

            Zeeshan Hamid and Andrea Armirotti

 

7. Lipidomics by HILIC-Ion Mobility-Mass Spectrometry

            Amy Li, Kelly M. Hines, and Libin Xu

 

8. A Workflow for the Identification of Mycotoxin Metabolites Using Liquid Chromatography–Ion Mobility-Mass Spectrometry

            Laura Righetti and Chiara Dall’Asta

 

9. Quantitation of Cyclosporin A in Cell Culture Media by Differential Mobility Mass Spectrometry (DMS-MS/MS)

            Amol Kafle, James Glick, Stephen L. Coy, and Paul Vouros

 

Part II: Proteomics and Glycomics

 

10. An Analytical Perspective on Protein Analysis and Discovery Proteomics by Ion Mobility-Mass Spectrometry

            Johannes P.C. Vissers and Michael McCullagh

 

11. Ion Mobility-Mass Spectrometry to Evaluate the Effects of Protein Modification or Small Molecule Binding on Protein Dynamics

            Lauren J. Tomlinson and Claire E. Eyers

 

12. Liquid Extraction Surface Analysis (LESA) High Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) Mass Spectrometry for In Situ Analysis of Intact Proteins

            Rian L. Griffiths, Klaudia I. Kocurek, and Helen J. Cooper

 

13. Ion Mobility-Mass Spectrometry of Glycoconjugates

            Weston B. Struwe and David J. Harvey

 

Part III: Ambient Ionization and Imaging Ion Mobility Mass Spectrometry

 

14. Desorption Atmospheric Pressure Photoionization Coupled with Ion Mobility-Mass Spectrometry

            Tiina J. Kauppila

 

15. Metabolomic Profiling of Adherent Mammalian Cells In Situ by LAESI-MS with Ion Mobility Separation

            Sylwia A. Stopka and Akos Vertes

 

16. Matrix-Assisted Laser Desorption and Desorption Electrospray Ionization Mass Spectrometry Coupled to Ion Mobility

            James I. Langridge and Emmanuelle Claude

 

Part IV: Bioinformatic Solutions for Analyzing IM-MS Data and Deriving CCS Values

 

17. The Use of LipidIMMS Analyzer for Lipid Identification in Ion Mobility-Mass Spectrometry-Based Untargeted Lipidomics

            Xi Chen, Zhiwei Zhou, and Zheng-Jiang Zhu

 

18. MolFind²: A Protocol for Acquiring and Integrating MS³ Data to Improve In-Silico Chemical Structure Elucidation for Metabolomics

            Milinda A. Samaraweera, Dennis W. Hill, and David F. Grant

 

19. Collision Cross Section Calculations Using HPCCS

            Gabriel Heerdt, Leandro Zanotto, Paulo C.T. Souza, Guido Araujo, and Munir S. Skaf

Blurb

This book focuses on ion mobility-mass spectrometry (IM-MS) and informatics approaches to improve traditional analysis of molecules by providing fundamentals and protocols for exploiting the potential of state-of-the-art IM-MS technology for the most common analytical applications. The chapters have been organized into four parts, each dealing with a particular set of IM-MS applications: 1) metabolomics and lipidomics; 2) proteomics and glycomics; 3) imaging and ambient ionization IM-MS; and 4) bioinformatic solutions for analyzing IM-MS data and deriving CCS values. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Ion Mobility-Mass Spectrometry: Methods and Protocols serves as an ideal resource for scientists delving into the technique's unprecedented analytical advantages, enabling novel qualitative and quantitative applications for the analysis of complex biological samples.