Intracellular Protein Catabolism II

ISBN-13: 9781461588153 / Angielski / Miękka / 2012 / 368 str.

Vito Turk

Opis

This book contains most of the papers presented . at the Second International Symposium on Intracellular Protein OItaboiism, which was held on May 26-30,1975 in Ljubljana, Yugoslavia. The papers presented in this book cover a wide variety of areas related to protein turnover and responsible enzymes. The symposium attracted about one hundred parti cipants, and the cordial atmosphere, the frank discussions and the exchange of ideas were a stimulant for all those attending_ During the last decade, investigations in the wide field of intracellular protein catabolism have been rapidly taken up in many laboratories, because the mechanisms and regulation of degradative processes are rather less studied compared to synthetic processes. In view of this increased interest, Prof. H. Hanson and his coworkers organized the First Interna tional Symposium and the papers presented there were published in the book Intracellular Protein Catabolism, edited by H. Hanson and P. Bohley. In the preface of this book they concluded: "We hope, however, the symposium as well as the book will be the beginning of a series of symposia and their publication concerning intracellular protein catabolism. " We would like to express our thanks for financial support provided by the Research Council of Slovenia. Special thanks are due to the organizing committee of the symposium, and to Dr. I. Kregar and particularly Dr. J. Babnik for their efforts in preparing manuscripts for the publishers."

Spis treści

Intracellular protein catabolism in vitro and in vivo..- An energy requirement for intralysosomal proteolysis in phagolysosomes and tissue slices..- Inhibition of cell protein degradation by microtubular inhibitors..- Increased degradation rates of canavanine-containing proteins in hepatoma cells..- Studies of the degradation of proteins in animal and bacterial cells..- Protein turnover in Neurospora crassa..- Studies on the mechanism of endocytosis in fibroblasts with the use of specific antibodies..- Specificity of breakdown based on the inactivation of active proteins and peptides by brain proteolytic enzymes..- Determination of the average degradation rate of mixtures of protein..- Primary reaction of intracellular protein catabolism..- Degradation of abnormal proteins in Escherichia coli: In vitro proteolysis of cyanogen bromide peptides..- Degradation of abnormal proteins in Echerichia coli: Susceptibility of CNBr generated peptides from alkaline phosphatase to proteolysis in E. coli cell exctracts..- Changes in metabolic stability of structural and enzyme proteins in experimental hepatomegaly..- Subcellular compart mentation and degradation of insulin in liver at the outset of hypoglicaemia induced by oral antidiabetic sulphanylureas..- Degradation of insulin and glucagon in homogenates and subcellular fractions of isolated pancreatic islets..- Heterogeneity of rat liver lysosomes with respect to proteolytic activity at pH 6.0..- The age dependence of intracellular proteolysis in the rat liver..- The breakdown of a tetrapeptide by yeast aminopeptidase I: Kinetic analysis and computer simulation..- Coton oil induced collagenolytic activity in the mouse skin..- Proteolytic degradation of insulin and glucagon by enzymes of rat liver..- Intracellular protein catabolism in muscle..- Protein turnover of rat skeletal muscle after denervation..- On the lysosomal role in skeletal muscle catabolism following denervation..- The importance of protein breakdown in muscle as a regulatory process..- Protein degradation in spontaneously degenerating muscles..- Separation of some peptide hydrolases from skeletal muscle..- Proteinases that digest the connective tissue matrix..- Cathepsins from experimental granuloma and their action on collagen..- Cathepsin D of rat thoracic duct lymphocytes: unusual intracellular localization and biochemical properties..- Purification and some properties of native and immobilized cathepsin D..- Isolation of cathepsin D by affinity chromatography on immobilized pepstatin..- Synthetic octapeptide inhibitor of cathepsin D..- Lung proteases and protease inhibitors..- The influence of cathepsin D inhibitors (pepstatin and anticatheptic antibodies on the serum cathepsin and lysozyme activity of 60Co irradiated animals..- The relationship between cathepsin D activity and antibacterial activity in subcellular fractions of rabbit and chicken polymorphonuclear leucocytes..- On the specificity of cathepsin B2..- A thiol dependent acid protease..- Cathepsin L and cathepsin B3 from rat liver lysosomes..- Activity of acid and neutral proteinases in bone marrow of busulfan treated rats..- The neutral proteinases of human spleen..- Isolation and some properties of horse leucocyte neutral proteases..- Neutral proteinases and inhibitors of leucocytes..- Neutral proteinases of bovine liver..- The catabolism of two adrenocorticotropin analogues following intravenous injection..- An autoradiographic study of the renal uptake and metabolism of a synthetic adrenocorticotrophin..- Appendix: Present knowledge of proteolytic enzymes and their inhibitors A. J. Barrett, I. Kregar, V. Turk, J. F. Woessner Jr..