Integrin and Cell Adhesion Molecules: Methods and Protocols

ISBN-13: 9781617791659 / Angielski / Twarda / 2011 / 529 str.

Motomu Shimaoka

Opis

Integrins play pivotal roles not only across a wide range of physiological processes including tissue morphogenesis, immune responses, wound healing, and regulation of cell growth and differentiation, but also in numerous pathological phenomena such as autoimmunity, thrombosis, and cancer metastasis/progression. Therefore, investigations on integrins often demand multi-disciplinary approaches, making researchers long for a handy collection of comprehensive and practical protocols that detail experimental methods for studying integrin and related cell adhesion molecule functionality. Integrin and Cell Adhesion Molecules: Methods and Protocols aims to provide readers not only with basic protocols in studying integrin functions, but also with summaries on those state-of-the-art technologies that have been utilized for understanding integrin functionality at the cellular, molecular, structural, and organismal levels. Divided into six convenient sections, this detailed volume covers basic protocols for the study of integrin and related cell adhesion molecule functionality in vitro, illustrates structural biology approaches for studying integrins and related cell adhesion molecules, focuses on emerging imaging technologies for investigating cell migration, presents strategies to elucidate signaling through cell adhesion molecules, includes experimental techniques to investigate integrin functions at organismal levels in a physiological context, and showcases the most promising methods and technologies for the development of novel therapeutics and diagnostics. Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Both experts and non-experts in the scientific community who wish to study cell adhesion molecules and diagnostics will find Integrin and Cell Adhesion Molecules: Methods and Protocols authoritative, easily accessible, and vastly informative.

Recenzje

From the reviews:

"This book provides an assortment of diverse topics for the study of adhesion molecules in vitro and in vivo, and as such it is laid out in a clear and accessible structure so that both experts and non-experts can use it as a reference for detailed protocols. ... appeal to a broad readership and contains sufficient depth in research background, detailed experimental protocols and relevant references to provide a valuable reference tool for PhD students as well as experienced researchers in this wide-ranging field." (Zania Stamataki, Immunology News, March, 2012)

Spis treści

Part I: Basic Protocols for the Study of Integrin and Related Cell Adhesion Molecule Functionality In Vitro

 

1. Overview: Assays for Studying Integrin-Dependent Cell Adhesion

            Alexandre Chigaev and Larry A. Sklar

 

2. Cell Adhesion Assays

            Gabriele Weitz-Schmidt and Stéphanie Chreng

 

3. Real Time Analysis of Integrin-dependent Transendothelial Migration and Integrin-independent Interstitial Motility of Leukocytes

            Ziv Shulman and Ronen Alon

 

4. Lentiviral Gene Transfer Method to Study Integrin Function in T Lymphocytes

            Daliya Banerjee and Motomu Shimaoka

 

5. Surface Plasmon Resonance Biosensing in Studies of the Binding between b2 Integrin I Domains and their Ligands

            Thomas Vorup-Jensen

 

6. Cell-Free Ligand-Binding Assays for Integrin LFA-1

            Koichi Yuki

 

 

Part II: Structural Biology Approaches for Studying Integrins and Related Cell Adhesion Molecules

 

7. Overview: Structural Biology of Integrins

            Guanyuan Fu, Wei Wang, and Bing-Hao Luo

 

8. Protein Expression and Purification of Integrin I Domains and IgSF Ligands for Crystallography

            Hongmin Zhang and Jia-huai Wang

 

9. Electron Microscopic Imaging of Integrin

            Kenji Iwasaki

 

10. An NMR Method to Study Protein-Protein Interactions

            Noritaka Nishida and Ichio Shimada

 

11. Single Molecule Methods to Study Cell Adhesion Molecules

            Joonil Seog

 

Part III: Imaging for Investigating Cell Adhesion and Migration

 

12. Overview: Imaging in the Study of Integrins

            Christopher V. Carman

 

13. Live Imaging of LFA-1-dependent T Cell Motility and Stop Signals

            Andrew J. Wiemer, Sarah Wernimont, and Anna Huttenlocher

 

14. Monitoring Integrin Activation by Fluorescence Resonance Energy Transfer

            Craig T. Lefort, Young-min Hyun, and Minsoo Kim

 

15. High-resolution Fluorescence Microscopy to Study Transendothelial Migration

            Christopher V. Carman

 

16. Multiphoton Intravital Microscopy to Study Lymphocyte Motility in Lymph Nodes

Thomas T. Murooka and Thorsten R. Mempel

 

Part IV: Signaling through Cell Adhesion Molecules

 

17. Overview of Integrin Signaling in the Immune System

            Tatsuo Kinashi

 

18. Rap1 and Integrin Inside-out Signaling

            Koko Katagiri and Tatsuo Kinashi

 

19. Isolation of Focal Adhesion Proteins for Biochemical and Proteomic Analysis

            Jean-Cheng Kuo, Xuemei Han, John R. Yates III, and Clare M. Waterman

 

20. Talin and Signaling Through Integrins

            Mohamed Bouaouina, David S. Harburger, and David A. Calderwood

 

21. Proteomics Method for Identification of Pseudopodium Phosphotyrosine Proteins

            Yingchun Wang and Richard L. Klemke

                       

Part V: Cell Adhesion and Migration at Organismal Levels in a Physiological Context

 

22. Overview: Studying Integrins In Vivo

            Clifford A. Lowell and Tanya N. Mayadas

 

23. A Method for the Generation of Conditional Gene-Targeted Mice

            Masahiro Yamamoto and Kiyoshi Takeda

 

24. T Cell Homing to the Gut Mucosa: General Concepts and Methodological Considerations

            Jaime De Calisto, Eduardo J. Villablanca, Sen Wang, Maria R. Bono, Mario Rosemblatt, and J. Rodrigo Mora

 

25. In Vivo Quantitative Proteomics: the SILAC-Mouse

            Sara Zanivan, Marcus Krueger, and Matthias Mann

 

26. Analysis of Chemotaxis in Dictyostelium

            Huaqing Cai, Chuan-Hsiang Huang, Peter N. Devreotes, and Miho Iijima

           

Part VI: Methods and Technologies towards Novel Therapeutics and Diagnostics Targeting Integrins

 

27. Integrins in Tumor Angiogenesis & Lymphangiogenesis

            Philippe Foubert and Judith A. Varner

 

28. PET-Radioimmunodetection of Integrins: Imaging Acute Colitis Using a 64Cu-Labeled Anti-b7 Integrin Antibody

            Jason L.J. Dearling and Alan B. Packard

 

29. Integrin-targeted Nanoparticles for siRNA Delivery

            Noa Ben-Arie, Ranit Kedmi, and Dan Peer

 

30. Humanized Mice for Studying Human Leukocyte Integrins In Vivo

            Sang-Soo Kim, Priti Kumar, and Premlata Shankar

Blurb

            Integrins play pivotal roles not only across a wide range of physiological processes including tissue morphogenesis, immune responses, wound healing, and regulation of cell growth and differentiation, but also in numerous pathological phenomena such as autoimmunity, thrombosis, and cancer metastasis/progression. Therefore, investigations on integrins often demand multi-disciplinary approaches, making researchers long for a handy collection of comprehensive and practical protocols that detail experimental methods for studying integrin and related cell adhesion molecule functionality. Integrin and Cell Adhesion Molecules: Methods and Protocols aims to provide readers not only with basic protocols in studying integrin functions, but also with summaries on those state-of-the-art technologies that have been utilized for understanding integrin functionality at the cellular, molecular, structural, and organismal levels. Divided into six convenient sections, this detailed volume covers  basic protocols for the study of integrin and related cell adhesion molecule functionality in vitro, illustrates structural biology approaches for studying integrins and related cell adhesion molecules, focuses on emerging imaging technologies for investigating cell migration, presents strategies to elucidate signaling through cell adhesion molecules, includes experimental techniques to investigate integrin functions at organismal levels in a physiological context, and showcases the most promising methods and technologies for the development of novel therapeutics and diagnostics. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

            Both experts and non-experts in the scientific community who wish to study cell adhesion molecules and diagnostics will find Integrin and Cell Adhesion Molecules: Methods and Protocols authoritative, easily accessible, and vastly informative.