In Situ Hybridization in Light Microscopy

ISBN-13: 9780849307034 / Angielski / Twarda / 2000 / 344 str.

Gerard Morel; Annie Cavalier

Opis

In Situ hybridization allows the visualization of specific DNA/RNA sequences in individual cells in tissue sections, single cells, or chromosome preparations, and is an especially important method for studying DNA and RNA in heterogeneous cell populations. This book delves into in situ hybridization methods through the use of light microscopy used by molecular biologists, pathologists, geneticists, and biochemists. It will also appeal to research scientists who are interested in visualizing methods for nucleic acids and proteins. The book features a two-column layout for protocols/principles, simplifies the techniques and offers many schemes and tables to help the reader choose the best procedure.

Spis treści

General Introduction
Abbreviations
Probes
Tissue Preparation
Pretreatments
Hybridization
Revelation of Hybrids
Counterstaining and Modes of Observation
Controls and Problems
Typical Protocols
Examples of Observation
Appendices
Glossary
Index

O autorze

Gerard Morel, Annie Cavalier