Immunoproteomics: Methods and Protocols

ISBN-13: 9781627035880 / Twarda / 2013 / 370 str.

Fulton, Kelly M.

Opis

Providing current diverse approaches and techniques used to study the immunoproteome, Immunoproteomics: Methods and Protocols collects chapters from key researchers that deliver information to be used in diagnostics, disease progression, and vaccine correlates of protection analysis, to name but a few. This detailed volume includes techniques used for the study of the antibody targets of bacterial pathogens, viruses, and cancer, mass spectrometry-based approaches to characterize T-cell epitopes, chapters on detection and relative quantification of cytokines in serum, as well as in silico prediction of epitopes using sequence-based or modeling approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and thorough, Immunoproteomics: Methods and Protocols aids researchers in transferring these techniques to their own laboratories in addition to providing a reference to guide researchers toward appropriate techniques.

Spis treści

1. Introduction to the Immune System

            Scott McComb, Aude Thiriot, Lakshmi Krishnan, and Felicity Stark

 

2. Immunoproteomics: Current Technology and Applications

            Kelly M. Fulton and Susan M. Twine

 

3. Antigen Identification Using SEREX

            Ugur Sahin and Özlem Türeci

 

4. Antigen Discovery Using Whole-Genome Phage Display Libraries

            Elisa Beghetto and Nicola Gargano

 

5. Methods and Applications of Serological Proteome Analysis

            Kelly M. Fulton, Shannon S. Martin, Lawrence Wolfraim, and Susan M. Twine

 

6. Pre-Absorbed Immunoproteomics: A Novel Method for the Detection of Bacterial Surface Proteins

            Guangjin Liu, Wei Zhang, and Chengping Lu

 

7. Identification of the Antigen Content of Electroimmunoprecipitates

            N. Helena Beyer and Niels H.H. Heegaard

 

8. An Immunoproteomics Approach to Screen the Antigenicity of the Influenza Virus

            Kevin M. Downard

 

9. In Vivo Microbial Antigen Discovery (InMAD) to Identify Diagnostic Proteins and Polysaccharides that Are Circulating During Microbial Infections

            Sindy J. Chaves, Kathleen Schegg, Thomas R. Kozel, and David P. AuCoin

 

10. Chemo-Enzymatic Production of O-Glycopeptides for the Detection of Serum Glycopeptide Antibodies

            Alexander Nøstdal and Hans H. Wandall

 

11. Enrichment and Characterization of Glycopeptide Epitopes from Complex Mixtures

            Luc Tessier, Kelly M. Fulton, and Susan M. Twine

 

12. Whole-Cell MALDI-TOF Mass Spectrometry: A Tool for Immune Cell Analysis and Characterization

            Richard Ouedraogo, Julien Textoris, Aurélie Daumas, Christian Capo, and Jean-Louis Mege

 

13. Cell-Based Arrays for the Identification of Interacting Polypeptide Domains or Epitopes

            Richard H. Maier, Christina J. Maier, and Kamil Önder

 

14. Identification and Quantitation of MHC Class II-Bound Peptides from Mouse Spleen Dendritic Cells by Immunoprecipitation and Mass Spectrometry Analysis

            Leonia Bozzacco and Haiqiang Yu

 

15. Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification

            Fei Wen and Huimin Zhao

 

16. Profiling of Cytokine and Chemokine Responses Using Multiplex Bead Array Technology

            Greg Harris and Wangxue Chen

 

17. Preparation of the Low Molecular Weight Serum Proteome for Mass Spectrometry Analysis

            Timothy J. Waybright, King C. Chan, Timothy D. Veenstra, and Zhen Xiao

 

18. Identification of Protein Biomarkers in Human Serum Using iTRAQ and Shotgun Mass Spectrometry

            Theodoros A. Koutroukides, Julian A.J. Jaros, Bob Amess, Daniel Martins-de-Souza, Paul C. Guest, Hassan Rahmoune, Yishai Levin, Mike Deery, Philip D. Charles, Svenja Hester, Arnoud Groen, Andy Christoforou, Julie Howard, Nick Bond, Sabine Bahn, and Kathryn S. Lilley

 

19. Genome-Based Bioinformatic Prediction of Major Histocompatibility Complex (MHC) Epitopes

            Simon J. Foote

 

20. Structure-Based Prediction of Major Histocompatibility Complex (MHC) Epitopes

            Andrew J. Bordner

 

21. Prioritization of Therapeutic Targets of Inflammation Using Proteomics, Bioinformatics, and In Silico Cell-Cell Interactomics

            Arsalan S. Haqqani and Danica B. Stanimirovic

 

22. Commercial Considerations for Immunoproteomics

            Scott M. Ferguson