Immunological investigation of autoimmune bullous dermatoses

ISBN-13: 9786208187873 / Angielski / Miękka / 2024 / 52 str.

Ameni JERBI

Opis

Autoimmune bullous dermatoses (ABD) are specific autoimmune diseases of the skin and mucous membranes. They are characterized by the presence of autoantibodies (auto-Ab) directed against skin and mucous membrane adhesion molecules, leading to altered function of their antigenic targets, resulting in clinical bullae and/or erosions. Depending on the site of cleavage, a distinction is made between intra-epidermal DBAI, characterized by auto-Ac directed against the structural proteins of the desmosomes that hold the keratinocytes of the epidermis together; and sub-epidermal DBAI, characterized by the presence of auto-Ac directed against the components of the dermo-epidermal junction (DEJ).The diagnosis of DBAI is based on clinical, histopathological and immunological evidence (detection of circulating or tissue-deposited auto-Ac). Direct immunofluorescence (DIF) is an immunological technique performed on cryosections of peri-lesional biopsies, based on the detection of Ac deposits and/or complement fractions using specific fluorescein-conjugated Ac.