Immunological Diagnosis of Leukemias and Lymphomas: International Symposium of the Institut Für Hämatologie, Gsf, October 28-30, 1976 -- Neuherberg/Mu

ISBN-13: 9783540082163 / Angielski / Miękka / 1977 / 390 str.

S. Thierfelder; H. Rodt; E. Thiel

Opis

This two-and-a-half-day symposium has concentrated on main aspects of the rapidly expanding field ofleukocyte markers in hematology. While leukemias are already being 'phenotyped' routinely in clinical centers, continued research on the developmental sta ge of cells and cell membranes, expanding into a malignant clone, permits new snap shots on hemopoietic differentiation. Thus the discovery of leukemia-associated anti gens, which so far have not been found on subpopulations of normal cells, has greatly stimulated the discussion on 'differentiation antigens versus tumor antigens'. The proceedings reflect the considerable success which has been achieved very re cently in the classification of hemoblastomas. Consequently the number of leukemias which are unclassifiable by immunological methods have dwindled down to a small mi nority. New facts give rise to new questions. By including the main points of the discussions in the proceedings, we wanted to give the reader an opportunity to get an impression of the questions and conclusions raised and drawn by the participants on the basis of new - and frequently still unpublished - data. The editors thank both the contributors to this symposium, who made it a successful meeting and submitted their manuscripts punctually, and the publishers, who have provided a volume of high quality in good time. They are also grateful for the valuable cooperation from numerous colleages at the Institut fUr Hamatologie."

Spis treści

1. Introductory Lecture.- The immunological diagnosis of human leukemias and lymphomas: an overview.- 2. Clinical significance of T and B cell surface markers in lymphoid malignancies.- Surface markers define human lymphoid malignancies with differing prognoses.- Cell markers and the classification of acute leukemia.- Etiological, clinical and prognostic significance of the T-B immunological classification of primary acute lymphoid leukemias and Non-Hodgkin’s lymphomas.- Surface receptors of human neoplastic lymphoreticular cells.- Non-Hodgkin lymphomas: cell populations and functional behavior.- 3. Leukemia-associated antigens versus cell differentation antigens in lymphatic and myeloic leukemias.- Membrane phenotyping: diagnosis, monitoring and classification of acute ‘lymphoid’ leukemias.- Cell differentiation antigens versus tumor-related antigens in childhood acute lymphoblastic leukemia (ALL). Clinical significance of leukemia markers.- Classification of leukemic cells with T- and O-ALL-specific antisera.- Membrane marker analysis of ‘lymphoid’ and myeloid blast crisis in Ph1 positive (chronic myeloid) leukemia.- Myeloid antigens and antigen densities in mice and men.- Expression of T and B cell markers and leukemia associated antigens on cell surface of human lymphocytic leukemias.- 4. Quantitative expression of surface markers on neoplastic lymphocytes.- Expression of FcIgG-receptor (FcIgG-r) sites on the surface of neoplastic lymphocytes.- Quantitation of T-antigenic sites and Ig-determinants on leukemic cells by microphotometric immunoradioautography. Proof of the clonal origin of thymus-derived lymphocytic leukemias.- Immunofluorometric analyses of membrane bound IgM in individual human lymphoid cells in cases of leukemia and malignant lymphoma.- 5. Characterization of leukemias and lymphomas by surface receptors and enzyme markers.- Hairy cell leukemia: simultaneous demonstration of autochthonous surface-Ig and monocytic functions of hairy cells.- Chronic lymphatic leukemia cells bear IgM receptors.- Acid phosphatase in acute lymphatic leukemia (ALL).- Lysosomal acid hydrolyses in isolated normal and chronic lymphocytic leukemia (CLL) T- and B-lymphocytes.- Prolymphocyte leukemia with T cell properties and tartrate resistant acid phosphatase.- Enzymecytochemical and immunocytological studies as a basis for the reevaluation of the histology of cutaneous B-cell and T-cell lymphomas.- Characterization of neoplastic mononuclear cells on tissue sections.- 6. Functional characteristics of neoplastic lymphocytes and/or of the resting lymphocyte population.- K cell activity of normal and chronic lymphocytic leukemia.- Comparative studies of the proportions of T- and B- and zero lymphocytes and the degree of blastic transformation of lymphocytes after stimulation by PHA and ConA in patients with Hodgkin’s disease.- In vitro functions of lymphoid cells in untreated childhood acute lymphoblastic leukemia.- Difference in distribution and dynamics of cellmarkers on T-lymphoblasts compared to T-lymphocytes.- Immunological evaluation of 15 children with Non-Hodgkin lymphoma.- Studies on the immune status of children with acute lymphocytic leukemia (ALL).- Lymphocyte subpopulations in lymphatic tissues and blood of patients with malignant lymphomas.- 7. Establishment and characterization of malignant lymphoid cell lines.- Establishment and characterization of leukemic T-cell lines, B-cell lines, and null-cell line: a progress report on surface antigen study of fresh lymphatic leukemias in man.- Established cell lines as tools in the study of human lymphoma and myeloma cell characteristics.- Characterization of ‘T’ and ‘non-T’ cell lines established from children with acute lymphoblastic leukemia and Non-Hodgkin lymphoma after leukemic transformation.- Establishment and characterization of a truly leukemic cell line.- Characterization of a human Hodgkin cell line and a lymphoblastic EBNA-negative cell line derived from a Non-Hodgkin lymphoma patient.- Autoradiographic detection of the Epstein-Barr virus (EBV) — associated early antigen (EA) in lymphoblastoid cell lines.- Lymphoid cell lines: in vitro cell markers in correlation to tumorigenicity in nude mice.- 8. Genetic markers define the mode of origin of lymphoid malignancies.- Glucose-6-phosphate dehydrogenase (G-6-PD) markers in Burkitt lymphoma and other malignancies.- The significance of chromosomal findings for the differentiation between lymphoma and lymphoblastoid cell lines.- Isoelectric focusing pattern of IgM and surface markers in Non-Hodgkin’s lymphomas..- Idiotypic determinants and antiglobulins in multiple myeloma.- 9. Ia determinants, blood group antigens and other markers for characterization of leukemic cell types.- New aspects in the classification of leukemic cell types by la alloantigens and complement receptors.- Heterologous group specific antiserum against Iak determinants.- The autoantigen determinants I/i, Pr1–3 of normal and leukemic leucocytes.- Alterations of blood group receptors of erythrocytes in leukemia — Immunofluorescence studies.- Two antibodies in anti-human T-cell sera.- Isolation of antilymphocyte antibodies with a solid waterinsoluble not gel-like immunoadsorbent.- Cationic proteins of leucocyte lysosomes: marker antigens for the immunological diagnosis of acute leukemias.- 10. Summing up.- Author Index.- Correspondence List.