Immune Receptors: Methods and Protocols

ISBN-13: 9781493957989 / Angielski / Miękka / 2016 / 301 str.

Jonathan P. Rast; James W. D. Booth

Opis

This book presents technical descriptions and protocols for a variety of experimental approaches to the characterization of immune receptors and the cell biology that mediates their functions. Written in the successful Methods in Molecular Biology(TM) format.

Spis treści

1. Screening for Ligands of C-Type Lectin-Like Receptors

            Elwira Pyż and Gordon D. Brown

 

2. Yeast Surface Display of Lamprey Variable Lymphocyte Receptors (VLR)

            Gang Xu, Satoshi Tasumi, and Zeev Pancer

 

3. Identification of Scavenger Receptor Ligands

            Claudine Neyen, Annette Pluddemann, and Siamon Gordon

 

4. Construction, Expression, and Purification of Chimeric Protein Reagents Based on Immunoglobulin Fc Regions

            John P. Cannon, Marci O’Driscoll, and Gary W. Litman

 

5. Innate Immune Receptors for Nucleic Acids

            Andrea Stutz, Damien Bertheloot, and Eicke Latz

 

6. Analysis of Receptor-Ligand Interactions by Surface Plasmon Resonance

            Kimiko Kuroki and Katsumi Maenaka

 

7. Cell-Based Reporter Assay to Analyze Activation of Nod1 and Nod2

            Birte Zurek, Harald Bielig, and Thomas A. Kufer

 

8. Determining FcεRI Diffusional Dynamics Via Single Quantum Dot Tracking

            Diane S. Lidke, Shalini T. Low-Nam, Patrick J. Cutler, and Keith A. Lidke

 

9. Ratiometric Analysis of Subcellular Recruitment of Fc Receptors During Phagocytosis

            Patricia Mero and James W.D. Booth

 

10. Assessment of the Recycling of the Membrane-Bound Chemokine, CX₃CL1

            Sajedabanu Patel, Ilya Mukovozov, and Lisa A. Robinson

 

11. Measuring Immune Receptor Mobility by Fluorescence Recovery after Photobleaching

            Kristen Silver and Rene E. Harrison

 

12. Probing the Plasma Membrane Structure of Immune Cells through the Analysis of Membrane Sheets by Electron Microscopy

            Björn F. Lillemeier and Mark M. Davis

 

13. Rapamycin-Based Inducible Translocation Systems for Studying Phagocytosis

            Michal Bohdanowicz and Gregory D. Fairn

 

14. Micropatterned Ligand Arrays to Study Spatial Regulation in Fc Receptor Signaling

            Alexis J. Torres, David Holowka, and Barbara A. Baird

 

15. CELLISA: Reporter Cell-Based Immunization and Screening of Hybridomas Specific for Cell Surface Antigens

            Peter Chen, Aruz Mesci, and James R. Carlyle

 

16. Use of Colloidal Silica-Beads for the Isolation of Cell-Surface Proteins for Mass Spectrometry-Based Proteomics

            Yunee Kim, Sarah Elschenbroich, Parveen Sharma, Lusia Sepiashvili, Anthony O. Gramolini, and Thomas Kislinger

 

17. Transfection-Based Genomic Readout for Identifying Rare Transcriptional Splice Variants

            Larry J. Dishaw, M. Gail Mueller, Robert N. Haire, and Gary W. Litman

 

18. Characterizing Somatic Hypermutation and Gene Conversion in the Chicken DT40 Cell System

            Nagarama Kothapalli and Sebastian D. Fugmann

 

19. Characterizing Immune Receptors from New Genome Sequences

            Katherine M. Buckley and Jonathan P. Rast

Blurb

Immunology has made significant progress in the past decade, driven forward by rapidly advancing technology and a renewed interest in the vast realm of innate immunity. The receptors that mediate these functions are at the front lines of both protective and regulative roles of the immune system.  In Immune Receptors: Methods and Protocols, expert researchers present a variety of experimental approaches to the characterization of immune receptors and the cell biology that mediates their functions.  These include imaging techniques that aim to understand receptor localization and trafficking, techniques to measure receptor-ligand interactions, strategies to identify novel ligands and methods to analyze downstream receptor signaling, as well as strategies for genomic and proteomic characterization of receptor repertoires. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Immune Receptors: Methods and Protocols offers technical descriptions and protocols that will be useful both to investigators who are interested in carrying out these procedures and to those who seek a deeper understanding of the bench science that lies behind the immunology literature.