Imaging and Spectroscopic Analysis of Living Cells: Optical and Spectroscopic Techniques Volume 504

ISBN-13: 9780123918574 / Angielski / Twarda / 2012 / 560 str.

P. Michael Conn

Opis

This volume of Methods in Enzymology is the first of three parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.

• Expert authors who are leaders in the field
• Extensively referenced and useful figures and tables
• Provides hints and tricks to facilitate reproduction of methods

Spis treści

Part A: Techniques

• Laser Induced Radiation Microbeam Technology and Simultaneous Real-time Fluorescence Imaging in Live Cells
• A cell biologist's guide to high resolution imaging
• Applications of fluorescence lifetime spectroscopy and imaging to lipid domains in vivo
• Detecting and Tracking Non-Fluorescent Nanoparticle Probes in Live Cells
• Fluorescence lifetime microscopy of tumor cell invasion, drug delivery and cytotoxicity
• Measuring membrane protein dynamics in neurons using Fluorescence recovery after photobleach (FRAP)
• Distinctive characteristics of Madeira wine regarding its traditional winemaking and modern analytical methodologies
• Green to red photo-convertible mEosFP aided live imaging in plants
• Methods for Cell and Particle Tracking
• Correlative light-electron microscopy: a potent tool for the imaging of rare or unique cellular and tissue events and structures
• Optical Techniques for Imaging Membrane Domains in Live Cells (Live Cell Palm of Protein Clustering)
• Single live cell topography and activity imaging with the Shear-Force-Based constant-distance scanning electrochemical microscope
• Visualization of TGN-endosome trafficking in mammalian and drosophila cells
• Live cell imaging with chemical specificity using dual frequency CARS microscopy
• Imaging intracellular protein dynamics by spinning disk confocal microscopy

Part B: Tools

• Visualizing Dynamic Activities of Signaling Enzymes using genetically encodable FRET-based biosensors: from designs to applications
• Live-cell Imaging of Aquaporin-4 Supramolecular Assembly and Diffusion
• Coiled-coil tag-probe labeling methods for live-cell imaging of membrane receptors
• Monitoring Protein Interactions in Living Cells with Fluorescence Lifetime Imaging Microscopy
• Open Source Tools for Fluorescent Imaging
• Nanoparticle PEBBLE sensors in live cells

O autorze

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. Conn is a previous member of Council for the American Society for Cell Biology and the Endocrine Society and is a prior President of the Endocrine Society, during which time he founded the Hormone Foundation and worked with political leadership to heighten the public's awareness of diabetes. Conn's students and fellows have gone on to become leaders in industry and academia. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science. He is the co-author of The Animal Research War (2008) and many articles for the public and academic community on the value of animal research and the dangers posed by animal extremism. His op/eds have appeared in The Washington Post, The LA Times, The Wall Street Journal, the Des Moines Register, and elsewhere. Conn consults with organizations that are influenced by animal extremism and with universities and companies facing challenges from these groups.