Imaging and Spectroscopic Analysis of Living Cells: Live Cell Imaging of Cellular Elements and Functions Volume 505

ISBN-13: 9780123884480 / Angielski / Twarda / 2012 / 576 str.

P. Michael Conn

Opis

This volume of Methods in Enzymology is the second of three parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.

• Expert authors who are leaders in the field
• Extensively referenced and useful figures and tables
• Provides hints and tricks to facilitate reproduction of methods

Spis treści

Part A: Organelles and Cellular Structure

• Monitoring cytoskeletal dynamics in living neurons using fluorescence photoactivation
• Use of DNA-specific anthraquinone dyes to directly reveal cytoplasmic and nuclear boundaries in live and fixed cells
• Methods to measure actin treadmilling rate in dendritic spines
• Dynamics of Clathrin coat formationImaging mitotic spindle dynamics
• Dynamics of microdomains
• Imaging chromosome dynamics in meiosis
• Analyzing lysosomes in live cells
• Imaging of mobile long-lived nanoplatforms in the live cell plasma membrane
• Visualizing ion channel dynamics at the plasma membrane
• Live imaging studies of the cytoskeleton
• Fluorescence single-molecule imaging of actin turnover and regulatory mechanisms
• Analysis of cell migration and cell-to-cell interaction by videomicroscopy

Part B: Molecules and Ions

• Real-time quantitative and qualitative assay for Glut-4 translocation
• Live-cell imaging with signalling complexes
• Dynamic imaging of oligomerization in living cells
• Time-resolved liminescence resonance energy transfer imaging of protein-protein interactions in living cells
• Imaging steriod receptors in live cells
• Investigating second messenger signaling in vivo
• Probes for intracellular RNA imaging in live cells
• Imaging the glycome in live cells
• Intracellular magnesium detection
• Imaging mobile zinc
• Imaging ion flux and ion homeostasis in live cells

O autorze

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. Conn is a previous member of Council for the American Society for Cell Biology and the Endocrine Society and is a prior President of the Endocrine Society, during which time he founded the Hormone Foundation and worked with political leadership to heighten the public's awareness of diabetes. Conn's students and fellows have gone on to become leaders in industry and academia. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science. He is the co-author of The Animal Research War (2008) and many articles for the public and academic community on the value of animal research and the dangers posed by animal extremism. His op/eds have appeared in The Washington Post, The LA Times, The Wall Street Journal, the Des Moines Register, and elsewhere. Conn consults with organizations that are influenced by animal extremism and with universities and companies facing challenges from these groups.