Imaging PET Reporter Transgenes in Living Subjects

ISBN-13: 9783639115178 / Angielski / Miękka / 2009 / 216 str.

Shahriar Yaghoubi

Opis

Non-invasive imaging of transgene expression shouldsoon play a critical role in gene and adoptivecellular gene therapy clinical trials. Positronemission tomography (PET) reporter genes (PRG)/probes(PRP) are currently the most advanced technologiesavailable for imaging transgens in humans. PRGs canbe used to image the location, magnitude, and timevariation of the intensity of transgene expressionthroughout the whole body of a patient. This caneliminate the need for multiple biopsies, which arepotentially dangerous and yet do not provide acomplete picture of whole body transgene expressionat multiple time points. This dissertation providesdetails of several studies on direct and indirectimaging of therapeutic transgenes using multiplePRG/PRP systems in mice. Furthermore, it includes thedetails of the first study on the pharmacokinetics,dosimetry, and safety of an imaging reporter probe inhumans. Finally, a complete background on alternatemethods of imaging transgene expression has beenprovided, concluding with a discussion of potentialfuture applications of imaging in gene therapy.

O autorze

Dr. Yaghoubi is currently a research scientist at the department
of Radiology, Molecular Imaging Program at Stanford University.
He received his Bachelors in Science in Biomedical Engineering
from University of Southern California in 1996 and his Ph.D. in
Molecular and Medical Pharmacology from University of California,
Los Angeles in 2002.