Hypertension: Methods and Protocols

ISBN-13: 9781617374937 / Angielski / Miękka / 2010 / 502 str.

Jerome P. Fennell; Andrew H. Baker

Opis

A collection of new and essential molecular techniques for cardiovascular research. These readily reproducible methods range widely from producing congenic, consomic, transgenic, and knockout models of hypertension to the gene transfer of specific genetic material using nonviral (polymers, liposomes, and antisense agents) and adenoviral vectors. Additional techniques described include single nucleotide polymorphism (SNP) genotyping, RNA interference, microarray analysis, pharmacogenetics, and pharmacogenomics for the genetic dissection of hypertension, as well as a practical method for deriving cardiomyocytes from embryonic stem cells that would serve as replacement cells for those damaged by hypertension or heart attack. The book offers both novice and experienced hypertension researchers an indispensable collection of readily reproducible techniques for successful research, work that has already dramatically improved the outlook for hypertensive patients, and promises much future success.

Recenzje

From the reviews:

"This is an outstandingly interesting book..." - Biomedical Chromatography

"This book of 29 chapters by 73 contributors is exclusively for researchers in hypertension." (American Journal of Cardiology, June, 2007)

Spis treści

Part I. Models of Hypertension Congenic/Consomic Models of Hypertension Delyth Graham, Martin W. McBride, Nicholas J. R. Brain, and Anna F. Dominiczak Mouse Knockout Models of Hypertension Michael Bader Production of Transgenic Models in Hypertension Elena Popova, Michael Bader, and Alexander Krivokharchenko Measuring Blood Pressure in Small Laboratory Animals Klaas Kramer and René Remie Part II. Assessment of Free Radicals in Endothelial Dysfunction Analysis of Superoxide Anion Production in Tissue Michela Zanetti, Livius V. d'Uscio, Timothy E. Peterson, Zvonimir S. Katusic, and Timothy O'Brien Measurement of Vascular Reactive Oxygen Species Production by Chemiluminescence Tomasz J. Guzik and Keith M. Channon A Guide to Wire Myography Angela Spiers and Neal Padmanabhan Part III. Nucleic Acid Techniques Selection of Candidate Genes in Hypertension Charles A. Mein, Mark J. Caulfield, and Patricia B. Munroe Genome-Wide Scanning With SSLPs in the Rat Carol Moreno, Kathleen Kennedy, Jaime Wendt Andrae, and Howard J. Jacob Extraction of RNA From Cells and Tissue Ian M. Bird Single-Strand Conformational Polymorphism Analysis: Basic Principles and Routine Practice Yanbin Dong and Haidong Zhu Single-Nucleotide Polymorphism Genotyping for Disease Association Studies Myriam Fornage and Peter A. Doris Denaturing High-Performance Liquid Chromatography Using the WAVE DNA Fragment Analysis System Elizabeth Donohoe TaqMan Real-Time PCR Quantification: Conventional and Modified Methods Michihiro Yoshimura, Shota Nakamura, and Hisao Ogawa Generation of High-Sensitivity Antisense cDNA Probes by Asymmetric PCR Ian M. Bird New Advances in Microarrays: Finding the Genes Causally Involved in Disease ClaudioNapoli, Filomena de Nigris, and Vincenzo Sica Pharmacogenetics and Pharmacogenomics Klaus Lindpaintner Part IV. Proteins and Proteomics Determination of the Endogenous Nitric Oxide Synthase Inhibitor Asymmetric Dimethylarginine in Biological Samples by HPLC Tom Teerlink Proteomic Approaches in the Analysis of Hypertension Soren Naaby-Hansen, Gayathri D. Warnasuriya, Claire Hastie, Piers Gallney, and Rainer Cramer Part V. Gene Transfer Gene Transfer in Endothelial Dysfunction and Hypertension Sabyasachi Sen, Padraig M. Strappe, and Timothy O'Brien Nonviral Gene Delivery Methods in Cardiovascular Diseases Marika Ruponen, Zanna Hyvönen, Arto Urtti, and Seppo Ylä-Herttuala Construction and Characterization of Helper-Dependent Adenoviral Vectors for Sustained In Vivo Gene Therapy Kazuhiro Oka and Lawrence Chan Adenovirus-Mediated Gene Transfer In Vivo: An Approach to Reduce Oxidative Stress Yi Chu Gene Therapy for Hypertension: Antisense Inhibition of the Renin-Angiotensin System M. Ian Phillips and Birgitta Kimura Modulation of Gene Expression by RNAi Cezary Wójcik, Rosalind Fabunmi, and George N. DeMartino In Vivo Biopanning: A Methodological Approach to Identifying Novel Targeting Ligands for Delivery of Biological Agents to the Vasculature Lorraine M. Work, Campbell G. Nicol, Laura Denby, and Andrew H. Baker Part VI. Stem Cells Cardiomyocytes Derived From Embryonic Stem Cells Kenneth R. Boheler, David G. Crider, Yelena Tarasova, and Victor A. Maltsev Part VII. Bioinformatics In Silico and Wet-Bench Identification of Nuclear Matrix Attachment Regions Stephen A. Krawetz, Sorin Draghici, Robert Goodrich, Zhandong Liu, and G. Charles Ostermeier Biomedical Informatics Methods in Pharmacogenomics Qing Yan Index

Blurb

Our understanding of the pathogenesis of hypertension and the development of new therapies for its management have each proven to be directly dependent on the availability and application of novel molecular biological methodologies. In Hypertension: Methods and Protocols, a panel of recognized experts from key institutions around the world describes in step-by-step detail many new and essential molecular techniques for cardiovascular hypertension research. The methods presented range widely from producing congenic, consomic, transgenic, and knockout models of hypertension to the gene transfer of specific genetic material using adenoviral and nonviral (polymers, liposomes, and antisense agents) vectors. Additional techniques described include single nucleotide polymorphism (SNP) genotyping, RNA interference, microarray analysis, pharmacogenetics, and pharmacogenomics for the genetic dissection of hypertension, as well as a practical method for deriving cardiomyocytes from embryonic stem cells that would serve as replacement cells for those damaged by hypertension or heart attack. A review of bioinformatic resources for pharmacogenomics describes the application of in silico strategies to identify nuclear matrix attachment regions. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Hypertension: Methods and Protocols offers both novice and experienced hypertension researchers an indispensable collection of readily reproducible techniques for successful research, work that has already dramatically improved the outlook for hypertensive patients, and promises much future success.