Highly selective OFF-ON fluorescent sensor for D-3-HB in aqueous

ISBN-13: 9783659953675 / Angielski / Miękka / 2016 / 56 str.

Qingming Wang

Opis

Diabetic ketoacidosis ( DKA ) is the most common hyper-glycemic emergency in patients with diabetes, and it was caused by too much ketones. Among the total ketone body, D-3-hydroxybutyric acid ( D-3-HB ) is the major, nearly 78%. So the detection of serum D-3-HB has been a routine marker for diagnosis of diabetic ketoacidosis. Fluorescent detection is the most efficient method, operational simplicity, low cost, real time monitoring and high sensitivity. Here, Two novel probe, 4-(2-hydroxybenzylicene)thiosemicarbazide ( 1 ) and 4-(1H- imidazo[4,5-f][1,10]- phenanthrolin-2-yl)phenol ( 2 ), were synthesized and shown a peculiar OFF-ON fluorescent response to D-3-HB in aqueous. The probe 1 & 2 have exhibited a selective and sensitive response to the D-3-HB over other common metal ions, anions and amino acids. Moreover the mechanism was confirmed by 1H-NMR and ESI-MS. Furthermore, this probe 1 successfully applied to bioimaging, demonstrating its value of practical applications in biological systems.

O autorze

Qingming Wang was born in Jiangsu,China, in 1984. He received his PhD in 2012 from Shanxi University. Subsequently he became an associate professor in Yancheng Teachers' University, China. His recent research focuses on the synthesis of optical probes based on fluorescein, coumarin, chromene, supramolecular chemistry and chiral molecules.