High-Throughput Screening Assays in Toxicology

ISBN-13: 9781071622155 / Angielski

Hao Zhu; Menghang Xia

Opis

This second edition volume expands on the previous edition by exploring the latest advancements in high throughput screening (HTS) in toxicity studies by using in vitro, ex vivo, and in vivo models. This volume also covers the application of artificial intelligence (AI) and data science to curate, manage, and use HTS data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and thorough, High Throughput Screening Assays in Modern Toxicology, Second Edition is a valuable resource for scientists pursuing chemical toxicology research. This book will aid scientists and researchers in translating new HTS techniques into standardized chemical toxicology assessment tools that can refine, reduce, and replace animal testing. 

Spis treści

Acknowledgement…

Preface…

Table of Contents…

Contributing Authors…

Part I In Vitro Toxicological High Throughput Screening Methods

1. Cell-Based Assays to Identify ERR and ERR/PGC Modulators

Caitlin Lynch, Jinghua Zhao, and Menghang Xia

2. Mitochondrial Membrane Potential Assay

Srilatha Sakamuru, Jinghua Zhao, Matias S. Attene-Ramos, and Menghang Xia

3. Cell-Based hERG Inhibition Assay in a High-Throughput Format

Jinghua Zhao, and Menghang Xia 

4. Identifying CAR Modulators Utilizing a Reporter Gene Assay

Caitlin Lynch, Jinghua Zhao, Hongbing Wang, and Menghang Xia

5. Study Liver Cytochrome P450 3A4 Inhibition and Hepatotoxicity Using DMSO-Differentiated HuH-7 Cells

Yitong Liu

6. Acetylcholinesterase Inhibition Assays for High-Throughput Screening

Shuaizhang Li, Andrew J. Li, Michael F. Santillo, and Menghang Xia

7. Cell-Based Assays to Identify Modulators of Nrf2/ARE Pathway

Zhengxi Wei, Jinghua Zhao, Li Zhang, and Menghang Xia

Part II In Vitro Toxicological High Content Screening Methods

8. Cell-Based Imaging Assay for Detection of Phospholipdosis

Li Zhang, Shuaizhang Li, and Menghang Xia

9. GFP-LC3 High-Content Assay for Screening Autophagy Modulators

Li Zhang, Jinghua Zhao, Wen-Xing Ding, and Menghang Xia

Part III Three-Dimensional Cell System for Toxicological High Throughput Screening

10. Generation of iPSC-Derived Brain Organoids for Drug Testing and Toxicological Evaluation

Ha Nam Nguyen

Part IV In Vivo Toxicological High Throughput Screening Methods

11. Zebrafish Behavioral Assays in Toxicology

Subham Dasgupta, Michael T. Simonich, and Robert L. Tanguay

Part V In Silico High Throughput Screening Toxicity Data Analysis

12. High Throughput Screening Assay Profiling for Large Chemical Databases

Daniel P. Russo and Hao Zhu

13. A Quantitative High Throughput Screening Data Analysis Pipeline for Activity Profiling

Ruili Huang

14. CurveP Method for Rendering High Throughput Screening Dose-Response Data into Digital Fingerprints

Alexander Sedykh

15. Accounting for Artifacts in High Throughput Toxicity Assays

Jui-Hua Hsieh

16. Automatic Quantitative Structure-Activity Relationship Modeling to Fill Data Gaps in High-Throughput Screening

Heather L. Ciallella, Elena Chung, Daniel P. Russo, and Hao Zhu

17. Use In Silico and In Vitro Methods to Screen Hepatotoxic Chemicals and CYP450 Enzyme Inhibitors

Yitong Liu

Subject Index List…