Heterologous Expression of Membrane Proteins: Methods and Protocols

ISBN-13: 9781493936359 / Angielski / Twarda / 2016 / 322 str.

Isabelle Mus-Veteau

Opis

This detailed volume encompasses chapters from leading experts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functional production and stabilization of recombinant integral membrane proteins (IMPs). Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. This book sets out to aid researchers in rectifying this situation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Second Edition serves as an ideal guide for scientists attempting to delve deeper into the myriad unique IMP structures.
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Spis treści

1. Cell-Free Production of Membrane Proteins in Escherichia coli Lysates for Functional and Structural Studies

            Ralf-Bernhardt Rues, Erik Henrich, Coilin Boland, Martin Caffrey, and Frank Bernhard

 

2. Membrane Protein Production in the Yeast, S. cerevisiae

            Stephanie P. Cartwright, Lina Mikaliunaite, and Roslyn M. Bill

 

3. Membrane Proteins Production in Escherichia coli: Protocols and Rules

            Federica Angius, Oana Ilioaia, Marc Uzan, and Bruno Miroux

 

4. Codon Optimizing for Increased Membrane Protein Production: A Minimalist Approach

            Kiavash Mirzadeh, Stephen Toddo, Morten H.H. Nørholm, and Daniel O. Daley

 

5. Generation of Tetracycline-Inducible Mammalian Cell Lines by Flow Cytometry for Improved Overproduction of Membrane Proteins

            Juni Andréll, Patricia C. Edwards, Fan Zhang, Maria Daly, and Christopher G. Tate

 

6. Membrane Protein Production in Lactococcus lactis for Functional Studies

            Daphne Seigneurin-Berny, Martin S. King, Emiline Sautron, Lucas Moyet, Patrice Catty, François André, Norbert Rolland, Edmund R.S. Kunji, and Annie Frelet-Barrand

 

7. Expression of Viral Envelope Glycoproteins in Drosophila melanogaster S2 Cells

            Renato Mancini Astray, Sandra Fernanda Suárez-Patiño, and Soraia Attie Calil Jorge

 

8. Leishmania tarentolæ as a Promising Tool for Expressing Polytopic and Multi-Transmembrane Spans Eukaryotic Membrane Proteins: The Case of the ABC Pump ABCG6

            Lucia Gonzalez-Lobato, Vincent Chaptal, Jennifer Molle, and Pierre Falson

 

9. Overexpression, Membrane Preparation, and Purification of a Typical Multidrug ABC Transporter BmrA

            Benjamin Wiseman and Jean-Michel Jault

 

10. Expression of Eukaryotic Membrane Proteins in Pichia pastoris

            Lucie Hartmann, Valérie Kugler, and Renaud Wagner

 

11. Integral Membrane Protein Expression in Saccharomyces cerevisiae

            Rebba C. Boswell-Casteel, Jennifer M. Johnson, Robert M. Stroud, and Franklin A. Hays

 

12. High-Throughput Baculovirus Expression System for Membrane Protein Production

            Ravi C. Kalathur, Marinela Panganiban, and Renato Bruni

 

13. Small-Scale Screening to Large-Scale Over-Expression of Human Membrane Proteins for Structural Studies

            Sarika Chaudhary, Sukanya Saha, Sobrahani Thamminana, and Robert M. Stroud

 

14. Purification of Human and Mammalian Membrane Proteins Expressed in Xenopus laevis Frog Oocytes for Structural Studies

            Rajendra Boggavarapu, Stephan Hirschi, Daniel Harder, Marcel Meury, Zöhre Ucurum, Marc J. Bergeron, and Dimitrios Fotiadis

 

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16. Detergent-Free Membrane Protein Purification

            Alice J. Rothnie

 

17. Conformational Dynamics and Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry

            Eric Forest and Petr Man

 

18. Lessons from an a-Helical Membrane Enzyme: Expression, Purification, and Detergent Optimization for Biophysical and Structural Characterization

            Jennifer L. Johnson, Sibel Kalyoncu, and Raquel L. Lieberman

 

19. Method to Screen Multidrug Transport Inhibitors Using Yeast Over-Expressing a Human MDR Transporter

            Laura Fiorini and Isabelle Mus-Veteau