Helicase Enzymes Part a: Volume 672

ISBN-13: 9780323914765 / Angielski / Twarda / 2022

Michael Trakselis

Spis treści

1. Fluorescence loading assay for hexameric helicases James Berger 2. Use of substrates with multiple fluorophores to simultaneously monitor unwinding of multiple duplexes Alicia Byrd 3. In silico reconstitution of MCM helicase loading and activation using time resolved cryo-EM Alessandro Costa 4. Helicase uncoupling to study nacent strand decay James Dewar 5. Investigating structure and dynamics of Twinkle helicase with Cryo-EM and high-speed AFM Yang Gao 6. Measuring helicase contributions to BIR Greg Ira 7. Crosslinking to define helicase/DNA interactions James L. Keck? 8. Use of Reverse Polarity DNA to investigate Helicase Mechanisms Tim Lohman 9. Substrate production to study helicases and translocases using molecular and optical tweezers Fernando Moreno Herrero 10. Methods of loading CMG onto DNA Michael O'Donnell 11. Methods to study Helicase-Polymerase coupling during DNA replication reactions Smita Patel 12. Biochemical analyses of ATP-dependent chromatin remodeling Craig Peterson 13. Alignment of helicases on single-stranded increases activity Kevin D. Raney 14. CMG helicase activity on G4-containing templates Dirk Remus 15. Accessory helicase unwinding of genomic complexities Grant Schauer 16. Monitoring helicase decoupling in bacteria by TUNEL Michael Trakselis 17. the preparation of DNA constructs for the use in single-molecule replisome studies Antoine Van Oijen 18. Interaction of topoisomerases with DNA measured by RADAR Alessandro Vindigni

O autorze

Michael grew up in a western suburb of Chicago called Aurora, IL, or better known as Wayne's World. He got his BS in Psychology and Chemistry at the University of Illinois, Urbana-Champaign. After which, he employed as a technician working on analytical method development at Abbott Laboratories. From 1998-2002 he was a PhD student at Penn State University working in Stephen Benkovic lab on the Bacteriophage T4 replisome assembly and dynamics. In 2003, he started a postdoctoral fellowship in Ron Laskey's laboratory at the University of Cambridge/Hutchison MRC in the UK. In 2006, he started his independent career at the University of Pittsburgh Chemistry department. Starting 2014, he moved his laboratory to Baylor University, where he currently lives with his family. There, the Trakselis laboratory is currently studying aspects of DNA replication and repair spanning from the very in vitro biochemical to the very in vivo cell and human biology.