Handbook of Isoelectric Focusing and Proteomics: Volume 7

ISBN-13: 9780120887521 / Angielski / Twarda / 2005 / 334 str.

David Garfin; Satinder Ahuja

Opis

Isoelectric focusing (IEF) is a high-resolution, stand-alone technique that can be used as an analytical method or tool for protein purification. The only current book on the market, the Handbook of Isoelectric Focusing and Proteomics is the ideal 'one-stop' source for germane information in this discipline. This highly practical book also contains chapters on alternative methods that may pave the way in the search for efficient techniques for fractionating and purifying proteins. Complete with the history of IEF focusing to authors' insights and practical tips, this book is a must for anyone working in proteomics.

* Is the only current book available on the subject
* Includes author insights and practical tips
* Is an ideal single source for students and researchers working in proteomics

Spis treści

Preface.

Incidents of Travel in IEF and IPGs.

Overview.

Evolution and Development of IEP.

Theory and Simulation of IEF.

Generation of pH Gradients.

Slab Gel IEF.

Two-Dimensional Gel Electrophoresis.

Some Practices and Pitfalls of Sample Preparation for IEF in Proeomics.

Protein Detection and Imaging in IEF Gels.

Capillary IEF.

Free Flow IEF.

Isoelectric Focusing and Proteomics.

Chromatofocusing.

Alternative Forms of IEF.

O autorze

Satinder Ahuja is a leading expert on water quality improvement. He earned his PhD in analytical chemistry from the University of the Sciences in Philadelphia. He worked for Novartis Corp. in various leadership positions for over 25 years and taught as an adjunct professor at Pace University for over 10 years. As president of Ahuja Consulting, he advises on water quality issues relating to chemicals and pharmaceuticals. A member of the executive committee of the Rivers of the World Foundation (ROW), Dr. Ahuja has organized numerous global symposia on improving water quality, including presentations for the American Chemical Society and UNESCO. Dr. Ahuja has published numerous papers and more than 25 books. His latest books are Contaminants in Our Water (ACS, 2020); Evaluating Water Quality to Prevent Future Disasters (Elsevier, 2019); Advances in Water Purification Techniques (Elsevier, 2019); and Chemistry and Water (Elsevier, 2017).