Handbook of Capillary Electrophoresis Applications

ISBN-13: 9789401071970 / Angielski / Miękka / 2011 / 738 str.

H. Shintani; J. Polonski

Opis

Over the last decade, high performance Capillary electrophoresis (HPCE) has emerged as a powerful and versatile separation technique that promises to rival high performance liquid chromatography when applied to the separation of both charged and neutral species. The high speed and high separation efficiency which can be attained using any of the various modes of HPCE has resulted in the increased use of the technique in a range of analytical environments. The procedures are, however, still in the early stages of development and several barriers remain to their adoption as the technique of choice for a range of analytical problems. One such barrier is the selection and optimization of the conditions required to achieve reproducible separations of analytes and it is in this area that this new book seeks to give assistance.
The book is written by an international team of authors, drawn from both academic and industrial users, and the manufacturers of instruments. At its heart are a number of tables, divided into specific application areas. These give details of published separations of a wide range of archetypal analytes, the successful separation conditions and the matrix in which they were presented. These tables are based on separations reported since 1992 and are fully referenced to the original literature. The tables are supported by discussions of the problems that a particular area presents and the strategies and solutions adopted to overcome them. The general areas covered are biochemistry, pharmaceutical science, bioscience, ion analysis, food analysis and environmental science.

Spis treści

Introduction. Part One: Equipment system: sample injection. quantitative analysis, precision and reproducibility. precision and quantitation in capillary electrophoresis. column technology. electrolyte systems. detection. recent advances in fluorescence detection technology. other systems. advanced systems. automated fraction collection in capillary electrophoresis. comparison with other analytical methods. PartTwo: Biochemitry applications: amino acids. sodium dodecyl sulfate(SDS)- non-gel molecular sieving capillary electrophoresis for proteins. protein analysis by capillary electrophoresis. analysis of erythropoietin by capillary electrophoresis. assay of enzymes by capillary electrophoresis. capillary electrophoresis based immunoassays. analysis of antibodies by capillary electrophoresis. analysis of peptide hormones and model peptides by capillary electrophoresis. affinity analysis by capillary electrophoresis. analysis of nucleic acids by capillary electrophoresis. Part Three: Pharmaceutical science applications: analysis of pharmaceuticals by capillary electrophoresis. analysis of medicinal plants: comparison of capillary electrophoresis with high performance liquid chromatography. quantitative applications of the resolution of enantiomers by capillary elecrophoresis. applications of chiral capillary electrophoresis (cyclodexedrin-capillary zone electrophoresis; CD-CZE). seperation of enantiomeric compounds by micelle-mediated capillary electrokenetic chromatography. therapeutic drug monitoring by capillary electrophoresis. Part Four: Bioscience applications: biomedical applications and biological systems. analysis of selected common clinical tests by capillary electrophoresis. gene analysis and nucleic acid sequencing. gene mutation and DNA sequencing. analysis of body fluids: urine, blood, saliva and tears. toxins associated with mainly uremia and cancer. Part Five: Ion analysis applications: capillary electrophoresis of metalcomplexes. metal chelation. inorganic ions. organic acids and organic ions. Part Six: Food analysis applications: food analysis by capillary electrophoresis. capillary zone electrophoresis analysis of additives in food samples. analysis of underivatized carbohydrateby capillary electrophoresis and electrochemical detection. Part Seven: Environmental science applications: hydrocarbons. environmental pollutants. analysis of synthetic polymers by capillary electro phoretic techniques. Appendix A: commercially available instrumentation for capillary electrophoresis. Appendix A1: overview of commercial instruments. Appendix A2: the CAPI series of instuments. Appendix B: troubleshooting. Appendix C: commercially available buffer reagents. Index.