Handbook of Assay Development in Drug Discovery

ISBN-13: 9781574444711 / Angielski / Twarda / 2006 / 488 str.

Lisa Minor; Minor K. Minor; Lisa K. Minor

Opis

Book Description With multiwell assays for drug discovery becoming a staple of the scientific community, the Handbook of Assay Development in Drug Discovery provides general guidelines and specific examples of each assay system. It includes radiometric assays, fluorescent-based assays, and reporter assays for such targets as kinases, proteases, nuclear receptors, and GPCRs. It presents descriptions of methods, exact protocols used to perform such methods, and troubleshooting tools. Featuring contributions from assay developers in the pharmaceutical industry as well as the vendor community, this text serves academics and discovery researchers developing and implementing assays.Shelving Guide: Contact Editor: OTI #1:

Recenzje

“To be as advanced as possible, Dr. Minor has had individual chapters written by experts from equipment vendors (GE, PerkinElmer); pharmaceutical companies (J&J, Merck, Lilly), government (NIH) and more. These individual chapters describe in practical terms what these organizations have developed.”
—Books-On-Line

Spis treści

Protein Kinases in Drug Discovery: Rationale, Success, and Challenge. An Introduction to the Protein Tyrosine Phosphatase Gene Family and Screening Assay Development. Time-Resolved Fluorescence and Time-Resolved Fluorescence Resonance Energy Transfer. Developement of High-Thoroughput Screening Assays for Kinase Drug Targets using AlphaScreen Technology. Homogeneous HTS Assays for Serine/Threonine Kinases using b-galactosidase Enzyme Fragment Complementation. IMAP Assays for Assaying Protein Kinases. A Homogeneous, Luminescent, High Throughput, and Versatile Assay for Wide Range of Kinases. Proteases as Drug Targets. Comparison of Homogeneous Bioluminescent and Fluorescent Methods for Protease Assays. SPA Receptor Binding Assays. Radioligand Binding Filtration Assay: Full Automation. Nuclear Receptors as Drug Targets. Nuclear Receptor Scintillation Proximity Assays. Homogeneous Assay Development for Nuclear Receptor using the AlphaScreen Technology. Development of Nuclear Receptor Homogeneous Assay using the Lance Technology. Emerging Role of Cell-based Assays in Discovery. The Preparation of Cells for High Content Screening. The Evolution of cAMP Assays. A Homogeneous Fluorescent Polarization Assay for Inositol 1, 4, 5 Trisphosphate (Ins P3). Scintillation Proximity Assay of Inositol Phosphates. Measuring Calcium Mobilization with Gq-Coupled GPCRs using the Fluorometric Imaging Plate Reader (FLIPR). Development of FLIPR-Based HTS Assay for Gi Coupled GPCRs. Aurora Assays. Membrane Potential Based Assays for Ion Channels and Electrogenic Transporters.

O autorze

Lisa K. Minor