Ghrelin

ISBN-13: 9780123812728 / Angielski / Twarda / 2012 / 512 str.

Masayasu Kojima

Opis

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The volume covers ghrelin, and has chapters on such topics as orphan gpcrs and methods for identifying their ligands, ghrelin o-acyltransferase assays and inhibition, and thermogenic characterization of ghrelin receptor null mice.

• Contains quality chapters authored by leaders in the field
• Has chapters on such topics as orphan gpcrs and methods for identifying their ligands, ghrelin o-acyltransferase assays and inhibition, and thermogenic characterization of ghrelin receptor null mice

Spis treści

1. History of GHS and ghrelin 2. History To The Discovery Of Ghrelin 3. Orphan Gpcrs And Methods For Identifying Their Ligands 4. Purification Of Rat And Human Ghrelins 5. Variety of Acyl Modifications in Mammalian Ghrelins 6. Determination Of Non-Mammalian Ghrelin

Molecular forms of ghrelin and Measuring of the concentrations 7. Morphological Analysis Of Ghrelin Neurons In The Hypothalamus 8. High-Performance Liquid Chromatography Analysis Of Hypothalamic Ghrelin 9. Standard Sample Collections For Blood Ghrelin Measurements

Enzymes for processing of ghrelin 10. From Ghrelin to Ghrelin's O Acyl Transferase 11. Enzymatic Characterization Of Goat, Ghrelin O-Acyltransferase 12. The Study Of Ghrelin Deacylation Enzymes

Synthesis of ghrelin agonists and antagonists 13. Synthesis Of Ghrelin Chemical Synthesis And Semi-Synthesis For Large-Scale Preparation Of Modified Peptides 14. Ghrelin O-Acyltransferase Assays And Inhibition

Action of ghrelin 15. Model-Based Evaluation Of Growth Hormone Secretion 16. Ghrelin In The Control Of Energy, Lipid And Glucose Metabolism 17. Ghrelin And The Vagus Nerve 18. Measurement of amp-activated protein kinase (ampk) activity and expression In response to ghrelin 19. Ghrelin And Gastrointestinal Movement 20. Ghrelin Acylation By Ingestion Of Medium-Chain Fatty Acids 21. Islet ß-cell Ghrelin Signaling For Inhibition Of Insulin Secretion 22. Rikkunshito As A Ghrelin Enhancer

Tg and KO mice of ghrelin 23. Thermogenic Characterization Of Ghrelin Receptor Null Mice 24. Transgenic Mice Over-Expressing Ghrelin Or Ghrelin Analog

Clinical application of ghrelin 25. Therapeutic Potential Of Ghrelin In Restricting-Type Anorexia Nervosa 26. Clinical Application Of Ghrelin For Chronic Respiratory Diseases 27. Clinical Trial Of Ghrelin Synthesis Administration For Upper Gi Surgery