Gene Transfer: Delivery and Expression of DNA and Rna, a Laboratory Manual

ISBN-13: 9780879697648 / Angielski / Twarda / 2006 / 793 str.

Theodore Friedmann; John J. Rossi

Opis

Understanding gene function and regulation requires rigorous testign in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. The vast number of avaiable techniques for clinical and laboratory research often makes selecting the optimal method a difficult process. Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. The detailed, step-by-step protocols cover a variety of methods, both well established and newly evolving. These include viral and nonviral methods of gene delivery, trans genic approaches, strategies for the regulation of trransgene expression, and modification of the host response. The introductory matter to each chapter includes concise technical and theoretical discussions wioth considerations for selection of appropriate system and strategies for delivery.