Gangliosides: Methods and Protocols

ISBN-13: 9781493993239 / Angielski / Miękka / 2019 / 456 str.

Sandro Sonnino; Alessandro Prinetti

Spis treści

1. Chemical and Physicochemical Properties of Gangliosides

            Laura Mauri, Sandro Sonnino, and Alessandro Prinetti

 

2. Gangliosides of the Nervous System

            Robert Ledeen and Gusheng Wu

 

3. Scattering Techniques and Ganglioside Aggregates: Laser Light, Neutron and X-Ray Scattering

            Elena Del Favero, Paola Brocca, and Laura Cantù

 

4. Gangliosides in the Immune System: Role of Glycosphingolipids and Glycosphingolipid-Enriched Lipid Rafts in Immunological Functions

            Kazuhisa Iwabuchi

 

5. Ganglioside Metabolism and Its Inherited Diseases

            Bernadette Breiden and Konrad Sandhoff

 

6. Gangliosides and Tumors

            Reiji Kannagi, Bi-He Cai, Hsiang-Chi Huang, Chia-Chun Chao, and Keiichiro Sakuma

 

7. Anti-Ganglioside Antibodies in Peripheral Nerve Pathology

            Hugh J. Willison

 

8. Protocols of Glycosyltransferase Assays: Ganglioside Globoside and Lewis-X Intermediate-Lactosylceramide Biosyntheses in Eukeryotic Systems

            Subhash Basu, Arun Agarwal, Manju Basu, Rui Ma, and Joseph R. Moskal

 

9. Mass Spectrometry of Gangliosides

            Akemi Suzuki, Minoru Suzuki, Emi Ito, Takahiro Nitta, and Jin-ichi Inokuchi

 

10. Visualization of Brain Gangliosides Using MALDI Imaging Mass Spectrometry

            Eiji Sugiyama and Mitsutoshi Setou

 

11. Immunoelectron Microscopy of Gangliosides

            Takuma Tsuji, Akikazu Fujita, and Toyoshi Fujimoto

 

12. Nuclear Magnetic Resonance of Gangliosides

            Domenico Acquotti, Laura Mauri, and Sandro Sonnino

 

13. Methods for the Preparation of Anti-Ganglioside Monoclonal Antibodies

            Noriyuki Yuasa and Ikuo Kawashima

 

14. Chemical Synthesis of Gangliosides

            Akihiro Imamura and Makoto Kiso

 

15. Radioactive Gangliosides for Biological Studies

            Laura Mauri, Simona Prioni, Maria Grazia Ciampa, and Sandro Sonnino

 

16. Synthesis of Fluorescent Gangliosides

            Günter Schwarzmann

 

17. Synthesis of Photoactivatable and Paramagnetic Gangliosides

            Günter Schwarzmann

 

18. Methods for Assay of Ganglioside Catabolic Enzymes

            Nicoletta Loberto, Giulia Lunghi, Domitilla Schiumarini, Maura Samarani, Elena Chiricozzi, and Massimo Aureli

 

19. Pharmacological Modulation of Glycosphingolipid Metabolism

            Jin-ichi Inokuchi, Takashi Ode, and Miki Hara-Yokoyama

 

20. Molecular Dynamics of Gangliosides

            Takumi Yamaguchi and Koichi Kato

 

21. Determination of Glycolipid Ligands of Carbohydrate-Binding Proteins Directly on Thin Layer Chromatograms

            John L. Magnani

 

22. Identification of KDN-Gangliosides

            Ken Kitajima

 

23. Glycoreplica Peptides

            Teruhiko Matsubara, Toshinori Sato, and Takao Taki

Blurb

This detailed volume explores methods of examining gangliosides, membrane amphiphilic lipids that are deeply involved in the modulation of the overall properties of cellular membranes, as well as of the functions of membrane-associated proteins. The contents take into account the vital availability of new enzymatic approaches, the knowledge of an incredibly extensive number of glyco-enzymes, and the improvement of multi-genetic analyses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Gangliosides: Methods and Protocols serves as a valuable guidebook for entering in the vast research world of gangliosides.