Galectins: Methods and Protocols

ISBN-13: 9781493952960 / Angielski / Miękka / 2016 / 488 str.

Sean R. Stowell; Richard D. Cummings

Opis

Galectins: Methods and Protocols is the first book solely dedicated to methodological approaches designed to study galectin function. The galectin family represents one of the most pleiotropic families, with individual members having been implicated in various aspects of nearly every biological process described, from RNA splicing to complex regulatory circuits that orchestrate adaptive immunity. Given the diverse roles of galectins in a variety of biological systems, studying these glycan binding proteins often requires the assimilation of diverse technical skills to fully appreciate their biological function. Their nearly ubiquitous expression and ability to bind highly modifiable carbohydrate ligands, in addition to a variety of other regulatory proteins, allows these glycan binding proteins (GBPs) to possess the capacity to regulate a wide variety of biological processes. Individual chapters are dedicated to examining salient features of galectin functions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Galectins: Methods and Protocols seeks to serve both professionals and novices with a useful framework when examining galectin function for many years to come.

Spis treści

1. Evolving Mechanistic Insights into Galectin Functions

Connie M. Arthur, Marcelo Dias-Baruffi, Richard D. Cummings, and Sean R. Stowell

 

2. Cloning, Expression and Purification of Galectins for In Vitro Studies

Paul A. Poland, Carol L Kinlough, and Rebecca P. Hughey

 

3. Alkylation of Galectin-1 with Iodoacetamide and Mass Spectrometric Mapping of the Sites of Incorporation

Sean R. Stowell, Connie M. Arthur, Richard D. Cummings, and Christa L. Feasley

 

4. Evaluation of Galectin Binding by Frontal Affinity Chromatography (FAC)

Jun Iwaki and Jun Hirabayashi

 

5. Probing Lectin-Mucin Interactions by Isothermal Titration Microcalorimetry

Tarun K. Dam and C. Fred Brewer

 

6. Examination of Whole Cell Galectin Binding by Solid Phase and Flow Cytometric Analysis

Anne Leppänen, Connie M. Arthur, Sean R. Stowell, Richard D. Cummings

 

7. Evaluation of Galectin Binding by Surface Plasmon Resonance

Padmaja Mehta-D’souza

 

8. Examining Galectin Binding Specificity Using Glycan Microarrays

Connie M. Arthur, Lilian C Rodrigues, Marcelo Dias-Baruffi, Harold C. Sullivan, Jamie Heimburg-Molinaro, Dave F. Smith, Richard D. Cummings, and Sean R. Stowell

 

9. Methods for Assessing the Effects of Galectins on Leukocyte Trafficking                      Beatrice R. Gittens, Rachael D. Wright, and Dianne Cooper

 

10. Examination of the Role of Galectins in Plasma Cell Differentiation

Chih-Ming Tsai and Kuo-I Lin

 

11. Examination of Galectin-Induced Lattice Formation on Early B-Cell Development

Stéphane J.C. Mancini, Latifa Elantak, Annie Boned, Marion Espéli, Françoise Guerlesquin, and Claudine Schiff

 

12. Detection of Phosophatidylserine Exposure on Leukocytes Following Treatment with Human Galectins

Connie M. Arthur, Lilian C Rodrigues, Marcelo Dias-Baruffi, Harold C. Sullivan Richard D. Cummings, and Sean R. Stowell

 

13. Examination of Galectins in Phagocytosis

Huan-Yuan Chen, I-Chun Weng, Chi-Shan Li, Lei Wan, and Fu-Tong Liu

 

14. Assessing the Roles of Galectins in Regulating Dendritic Cell Migration Through Extracellular Matrix and Across Lymphatic Endothelial Cells

Sandra Thiemann, Jeanette H. Man, and Linda G. Baum

 

15. Examination of the Role of Galectins in Intestinal Inflammation

Atsushi Nishida, Cindy W. Lau, and Atsushi Mizoguchi

 

16. Study of Galectins in Tumor Immunity: Strategies and Methods

Juan P. Cerliani, Tomas Dalotto-Moreno, Daniel Compagno, L. Sebastián Dergan-Dylon, Diego J. Laderach, Lucas Gentilini, Diego O. Croci, Santiago P. Méndez-Huergo, Marta A. Toscano, Mariana Salatino, and Gabriel A. Rabinovich

 

17. Galectins in the Regulation of Platelet Biology

            Albertina Romaniuk, Gabriel A Rabinovich, and Mirta Schattner

 

18. Examination of the Role of Galectins and Galectin Inhibitors in Endothelial Cell Biology

Iris A.E. Schulkens, Esther A. Kleibeuker, Kitty C.M. Castricum, Arjan W. Griffioen, and Victor L.J.L. Thijssen

 

19. Regulation of Galectins by Hypoxia and Their Relevance in Angiogenesis: Strategies and Methods

Mariana Salatino, Diego O. Croci, Diego J. Laderach, Daniel Compagno, Lucas Gentilini, Tomas Dalotto-Moreno, L. Sebastián Dergan-Dylon, Santiago P. Méndez-Huergo, Marta A. Toscano, Juan P. Cerliani and Gabriel A. Rabinovich

 

20. Examination of the Role of Galectins During In vivo Angiogenesis Using the Chick Chorioallantoic Membrane Assay

Esther A. Kleibeuker, Iris A. Schulkens, Kitty C.M. Castricum, Arjan W. Griffioen, Victor L.J.L. Thijssen

 

21. Examination of the Role of Galectins in Cell Migration and Re-Epithelialization of Wounds

Zhiyi Cao, Chandrassegar Saravanan, Wei-Sheng Chen, and Noorjahan Panjwani

 

22. Manipulating Galectin Expression in Zebrafish (Danio rerio)

Chiguang Feng, Mihai Nita-Lazar, Nuria González-Montalbán, Jingyu Wang, Justin Mancini, Chinnarajan Ravindran, Hafiz Ahmed, and Gerardo R. Vasta

 

23. Examination of Galectin Localization Using Confocal Microscopy

Daniel Giuliano Cerri, Connie M. Arthur, Lílian Cataldi Rodrigues, Marise Lopes Fermino, Lenaldo Branco Rocha, Sean R. Stowell, and Marcelo Dias-Baruffi

 

24. Examination of the Regulation of Galectin-3 Expression in Cancer

Hafiz Ahmed and Gargi Bandyopadhyaya

 

25. Evaluation of the Role of Galectins in Parasite Immunity

Sarah Preston, Jillian Dunphy, Travis Beddoe, Els Meeusen, and Anna Young

 

26. Effect of Galectins on Viral Transmission

Michel Ouellet, Christian St-Pierre, Michel J. Tremblay, and Sachiko Sato

 

27. Evaluation of the Bactericidal Activity of Galectins

Connie M. Arthur, Richard D. Cummings, and Sean R. Stowell

 

28. Examination of the Role of Galectins in Pre-mRNA Splicing

Ronald J. Patterson, Kevin C. Haudek, Patricia G. Voss, and John L. Wang

           

29. Analysis of the Intracellular Role of Galectins in Cell Growth and Apoptosis

Daniel K. Jsu, Ri-Yao Yang, Jun Saegusa, and Fu-Tong Liu

 

30. Nucleocytoplasmic Shuttling of Galectin-3

            Eric J. Arnoys, Cheri M. Ackerman, and John L. Wang