GPCR Signalling Complexes – Synthesis, Assembly, Trafficking and Specificity

ISBN-13: 9789401780995 / Angielski / MiÄ™kka / 2014 / 300 str.

Denis J. Dupre;Terence E. Hebert;Ralf Jockers

Opis

Main Question: G protein coupled receptors are involved in highly efficient and specific activation of signalling pathways. How do GPCR signalling complexes get assembled to generate such specificity? In order to answer this question, we need to understand how receptors and their signalling partners are synthesized, folded and quality-controlled in order to generate functional proteins. Then, we need to understand how each partner of the signalling complex is selected to join a complex, and what makes this assembly possible. GPCRs are known to be able to function as oligomers, what drives the assembly into oligomers and what will be the effects of such organization on specificity and efficacy of signal transduction. Once the receptor complexes are assembled, they need to reach different locations in the cell; what drives and controls the trafficking of GPCR signalling complexes. Finally, defects in synthesis, maturation or trafficking can alter functionality of GPCRs signalling complexes; how can we manipulate the system to make it function normally again? Pharmacological chaperones may just be part of the answer to this question.

Spis treści

1. ER-bound steps in the biosynthesis of G protein-coupled receptors; Christian Nanoff and Michael Freissmuth 

2. Role of chaperones in G protein coupled receptor signalling complex assembly; Denis J. Dupré, Maha M. Hammad, Patrick Holland and Jaime Wertman

3. GPCR oligomerization: contribution to receptor biogenesis: Kathleen Van Craenenbroeck

4. The functional size of GPCRs- monomers, dimers or tetramers?; Darlaine Pétrin and Terence E. Hébert

5. Regulation of post-Golgi traffic of G protein-coupled receptors; Guangyu Wu

 6. Regulated GPCR trafficking to the plasma membrane: general issues and the CCR5 chemokine receptor example; Hamasseh Shirvani, Gabriel Gätà and Stefano Marullo

7. Regulatory Processes Governing the Cell Surface Expression of LH and FSH Receptors; Deborah L. Segaloff

8. Chaperone-mediated assembly of G protein complexes; Barry M. Willardson and Christopher M. Tracy 

9. Synthesis and assembly of G protein bg dimers: comparison of in vitro and in vivo studies; Jane Dingus and John D. Hildebrandt 

10. Preferential assembly of G-abg complexes directed by the g subunits; Janet D. Robishaw

11. G Protein Trafficking; Philip B. Wedegaertner 

12. Differential assembly of GPCR signaling complexes determines signaling specificity; Pascal Maurice , Abla Benleulmi-Chaachoua & Ralf Jockers 

 13. GPCR and voltage-gated calcium channels (VGCC) signaling complexes; Christophe Altier 

14. Pharmacological chaperones correct misfolded GPCRs and rescue function: trafficking as a therapeutic target; Guadalupe Maya-Núñez, Alfredo Ulloa-Aguirre, Jo Ann Janovick, P. Michael Conn

Blurb

This book provides a broad survey of various topics pertaining to G protein coupled receptor (GPCR) assembly into specific signalling complexes, and the regulation of the events leading to this assembly. Throughout this book, we focus on one main area: GPCRs are involved in highly efficient and specific activation of signalling pathways; how are these signalling complexes assembled to generate such specificity? To address this issue, we need to understand how receptors and their signalling partners are synthesized, folded correctly and assembled in order to generate functional complexes. The effects of oligomerization on specificity and efficacy of signal transduction are also discussed. Once receptor complexes are assembled, how are they targeted to different intracellular sites and what controls the trafficking of GPCR signalling complexes? Finally, defects in synthesis, maturation or trafficking can alter functionality of GPCR signalling complexes; how can we manipulate these systems to normalize them functionally?  Therefore, the topics to be covered in this book are:

1) Molecular chaperones involved in regulating (quality control) of GPCR synthesis;
2) Assembly of signalling complexes, or subunits of the complex and their organization;
3) GPCR oligomerization;
4) GPCR trafficking;
5) Problems of trafficking: pharmacological chaperones to the rescue.