G Protein Pathways, Part B: G Proteins and Their Regulators: Volume 344

ISBN-13: 9780121822453 / Angielski / Twarda / 2001 / 813 str.

Ravi Iyengar; John D. Hildebrandt

Opis

This volume covers topics such as the structure and identification of functional domains of G proteins, and activation of G proteins by receptors or other regulators. The text takes an integrated approach to studying common experimental questions at many different levels related to G proteins. Methods related to G proteins using molecular modeling, systems biology, protein engineering, protein biochemistry, cell biology, and physiology are all accessible in the same volume.
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Recenzje

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Spis treści

Section I: Activation of G Proteins by Receptors or Other Regulators

[1]: Analysis of G Protein Activation in Sf9 and Mammalian Cells by Agonist-Promoted [³⁵S]GTP?S Binding

[2]: Elucidating Kinetic and Thermodynamic Constants for Interaction of G Protein Subunits and Receptors by Surface Plasmon Resonance Spectroscopy

[3]: Neuroanatomical Localization of Receptor-Activated G Proteins in Brain

[4]: Design and Use of C-Terminal Minigene Vectors for Studying Role of Heterotrimeric G Proteins

[5]: Dissecting Receptor-G Protein Specificity Using Ga Chimeras

[6]: Use of Dominant Negative Mutations in Analysis of G Protein Function in Saccharomyces cerevisiae

[7]: Functional Assays for Mammalian G-Protein-Coupled Receptors in Yeast

[8]: Role of G Protein ß? Complex in Receptor-G Protein Interaction

[9]: Phosducin Down-Regulation of G-Protein Coupling: Reconstitution of Phosducin Transducin of cGMP Cascade in Bovine Rod Photoreceptor Cells

[10]: Analysis of Signal Transfer from Receptor to G_o/G_i in Different Membrane Environments and Receptor-Independent Activators of Brain G Protein

[11]: Identification of Modulators of Mammalian G-Protein Signaling by Functional Screens in the Yeast Saccharomyces cerevisiae

Section II: Isolation or Production of Native or Modified

[12]: Expression of a Subunit of G_s in Escherichia coli

[13]: Purification of G Protein Isoforms G_OA G_OC from Bovine Brain

[14]: Coexpression of Proteins with Methionine Aminopeptidase/or N-Myristoyltransferase in Escherichia coli to Increase Acylation Homogeneity of Protein Preparations

[15]: Purification of G Protein ß? from Bovine Brain

[16]: Separation and Analysis of G Protein ? Subunits

[17]: Activity of G? Prenylcysteine Carboxyl Methyltransferase

[18]: Preparation and Application of G Protein ? Subunit-Derived Peptides Incorporating a Photoactive Isoprenoid

Section III: Functional Analysis of G Protein Subunits

[19]: Expression and Functional Analysis of G Protein a Subunits in S49 Lymphoma Cells

[20]: Mouse Gene Knockout Knockin Strategies in Application to a Subunits of G_i/G_o Family of G Proteins

[21]: Determining Cellular Role of Ga₁₂

[22]: Targeted, Regulatable Expression of Activated Heterotrimeric G Protein a Subunits in Transgenic Mice

[23]: Inducible, Tissue-Specific Suppression of Heterotrimeric G Protein a Subunits in Vivo

[24]: Construction of Replication Defective Adenovirus That Expresses Mutant Ga_s Q227L

[25]: Expression of Adenovirus-Directed Expression of Activated Ga_s in Rat Hippocampal Slices

[26]: Quench-Flow Kinetic Measurement of Individual Reactions of G-Protein-Catalyzed GTPase Cycle

[27]: Analysis of Genomic Imprinting of G_sa Gene

[28]: Subcellular Localization of G Protein Subunits

[29]: Fluorescence Approaches to Study G Protein Mechanisms

[30]: Defining G Protein ß? Specificity for Effector Recognition

[31]: Ribozyme-Mediated Suppression of G Protein ? Subunits

Section IV: G Protein Structure and Identification

[32]: Use of Scanning Mutagenesis to Delineate Structure-Function Relationships in G Protein a Subunits

[33]: Development of G_s-Selective Inhibitory Compounds

[34]: Characterization of Deamidated G Protein Subunits

[35]: Determining G Protein Heterotrimer Formation

[36]: Use of Peptide Probes to Determine Function of Interaction Sites in G Protein Interactions with Effectors

[37]: Protein Interaction Assays with G Proteins

[38]: Evolutionary Traces of Functional Surfaces along G Protein Signaling Pathway

[39]: Discovery of Ligands for ß? Subunits from Phage-Displayed Peptide Libraries

[40]: Exploring Protein-Protein Interactions by Peptide Docking Protocols

[41]: Structural Characterization of Intact G Protein ? Subunits by Mass Spectrometry

Section V: RGS Proteins and Signal Termination

[42]: Quantitative Assays for GTPase-Activating Proteins

[43]: Analysis of RGS Proteins in Saccharomyces cerevisiae

[44]: Purification of RGS Protein, Sst2, from Saccharomyces cerevisiae and Escherichia coli

[45]: RGS Domain: Production and Uses of Recombinant Protein

[46]: Screening for Interacting Partners for Ga_i3 and RGS-GAIP Using the Two-Hybrid System

[47]: Assay of RGS Protein Activity in Vitro Using Purified Components

[48]: Measuring RGS Protein Interactions with G_qa

[49]: Assays of Complex Formation between RGS Protein G? Subunit-like Domains and Gß Subunits

[50]: RGS Function in Visual Signal Transduction

[51]: Molecular Cloning of Regulators of G-Protein Signaling Family Members and Characterization of Binding Specificity of RGS 12 PDZ Domain

Author index

Subject Index