Fundamentals of Protein Structure and Function

ISBN-13: 9783319792903 / Angielski / Miękka / 2019 / 521 str.

Engelbert Buxbaum

Opis

This book serves as an introduction to protein structure and function. Starting with their makeup from simple building blocks, called amino acids, the 3-dimensional structure of proteins is explained. This leads to a discussion how misfolding of proteins causes diseases like cancer, various encephalopathies, or diabetes. Enzymology and modern concepts of enzyme kinetics are then introduced, taking into account the physiological, pharmacological and medical significance of this often neglected topic. This is followed by thorough coverage of haemoglobin and myoglobin, immunoproteins, motor proteins and movement, cell-cell interactions, molecular chaperones and chaperonins, transport of proteins to various cell compartments and solute transport across biological membranes. Proteins in the laboratory are also covered, including a detailed description of the purification and determination of proteins, as well as their characterisation for size and shape, structure and molecular interactions. The book emphasises the link between protein structure, physiological function and medical significance. This book can be used for graduate and advanced undergraduate classes covering protein structure and function and as an introductory text for researchers in protein biochemistry, molecular and cell biology, chemistry, biophysics, biomedicine and related courses. About the author: Dr. Buxbaum is a biochemist with interest in enzymology and protein science. He has been working on the biochemistry of membrane transport proteins for nearly thirty years and has taught courses in biochemistry and biomedicine at several universities.

Spis treści

Part I: Proteins.- Amino Acids.- Protein Structure.- Proteins in the Lab.- Part II: Enzymes.- Enzymes are Biocatalysts.- Enzyme Kinetics and Mechanism.- Inhibition and Inactivation of Enzymes.- Hæmyoglobin and Myoglobin: Cooperativity.- Enzyme Kinetics: Special Cases.- The Flow of Metabolites through Metabolic Pathways.- Part III: Special Proteins.- Protein Folding Diseases.- Immunoproteins.- Cell Skeleton.- Motor Proteins and Movement.- Cell-cell interactions.- Aiding in Folding: Molecular Chaperones and Chaperonins.- Part IV: Membrane Transport.- Protein Transport across Membranes.- Vesicular Transport in Eukaryotic Cells.- Transport of Solutes across Membranes.- Appendices.

O autorze

Dr. Buxbaum is a biochemist with interests in enzymology and protein science. He has special experience with isolation of soluble and membrane proteins, assay development, enzyme kinetics, binding, and transport studies. Dr. Buxbaum has taught courses in biochemistry, protein science, and molecular medicine at various universities.

Blurb

This book serves as an introduction to protein structure and function. Starting with their makeup from simple building blocks, called amino acids, the 3-dimensional structure of proteins is explained. This leads to a discussion of how misfolding of proteins causes diseases like cancer, various encephalopathies, or diabetes. Enzymology and modern concepts of enzyme kinetics are then introduced, taking into account the physiological, pharmacological, and medical significance of this often neglected topic. This is followed by thorough coverage of hæmoglobin and myoglobin, immunoproteins, motor proteins and movement, cell-cell interactions, molecular chaperones and chaperonins, transport of proteins to various cell compartments, and solute transport across biological membranes. Proteins in the laboratory are also covered, including a detailed description of  the purification and determination of proteins, as well as their characterisation for size and shape, structure, and molecular interactions. The book emphasises the link between protein structure, physiological function, and medical significance.

This book can be used for graduate and advanced undergraduate classes covering protein structure and function and as an introductory text for researchers in protein biochemistry, molecular and cell biology, chemistry, biophysics, biomedicine, and related courses.

About the author:

Dr. Buxbaum is a biochemist with an interest in enzymology and protein science. He has been working on the biochemistry of membrane transport proteins for nearly thirty years and has taught courses in biochemistry and biomedicine at several universities.