Functional Glycomics: Methods and Protocols

ISBN-13: 9781493956975 / Angielski / Miękka / 2016 / 284 str.

Jianjun Li

Opis

This reference provides the latest developments and emerging techniques used to study functional glycomics. Chapters included needed materials, reagants, step-by-step protocols, notes sections, common pitfalls to avoid and tips on troubleshooting.

Recenzje

From the reviews:

"This book provides a useful introduction to the field of glycobiology as well as detailed protocols relating to methods of analyses. ... relevance to the microbiologist who is considering exploring this field and wishes to gain an understanding of current methodologies." (Sheila Patrick, Microbiology Today, 2010)

"The book consists of 19 chapters prepared by an international team of authors. ... book will be very useful source for many researchers in glycobiology fields. It should be recommended also to students and teachers of colleges and universities who specialized in glycobiology. I would like to mention that the book is well done including a materials and methods part to each chapter, references, subject index, figures and tables." (G. Ya. Wiederschain, Biochemistry, Vol. 75 (11), 2010)

Spis treści

1. Functional Glycomics and Glycobiology: An Overview Jianjun Li and James C. Richards 2. Historical Overview of Glycoanalysis Alicia M. Bielik and Joseph Zaia 3. Quantitative Glycomics Ron Orlando 4. Human Gangliosides and Bacterial Lipo-oligosaccharides in the Development of Autoimmune Neuropathies Nobuhiro Yuki 5. Biotinylated Multivalent Glycoconjugates for Surface Coating Alexander Chinarev, Oxana Galanina, and Nicolai Bovin 6. Profiling LPS Glycoforms of Non-typeable Haemophilus influenzae by Multiple-Stage Tandem Mass Spectrometry Elke K.H. Schweda and James C. Richards 7. In vitro Reconstitution of Escherichia coli O86 O Antigen Repeating Unit Weiqing Han, Lei Li, Nicholas Pettit, Wen Yi, Robert Woodward, Xianwei Liu, Wanyi Guan, Veer Bhatt, Jing Katherine Song, and Peng George Wang 8. Glycoprotein Characterization Susan M. Twine, Luc Tessier, and John F. Kelly 9. N-Linked Glycoprotein Analysis Using Dual-Extraction Ultrahigh-Performance Liquid Chromatography and Electrospray Tandem Mass Spectrometry S. O. Siu, Maggie P. Y. Lam, Edward Lau, William S.B. Yeung, David M. Cox, and Ivan K. Chu 10. Microarray-Based Study of Carbohydrate-Protein Binding Zhenxin Wang and Jingqing Gao 11. The Application of NMR Spectroscopy to Functional Glycomics Jean-Robert Brisson, Evgeny Vinogradov, David McNally, Nam Huan Khieu, Ian Schoenhofen, Susan Logan, and Harold Jarrell 12. Metabolomics in Glycomics Evelyn C. Soo and Joseph P. M. Hui 13. Characterization of Lipid-Linked Oligosaccharides by Mass Spectrometry Christopher W. Reid, Jacek Stupak, and Christine M. Szymanski 14. A Glycomics Approach to the Discovery of Potential Cancer Biomarkers Hyun Joo An and Carlito B. Lebrilla 15. Extraction of Chondroitin/Dermatan Sulfate Glycosaminoglycans from Connective Tissue for Mass Spectrometric Analysis Alicia M. Bielik and Joseph Zaia 16. N-Linked Protein Glycosylation in a Bacterial System Harald Nothaft, Xin Liu, David J. McNally, and Christine M. Szymanski 17. Characterization of Polysaccharides Using Mass Spectrometry for Bacterial Serotyping Eleonora Altman and Jianjun Li 18. Is Permethylation Strategy Always Applicable to Protein N-Glycosylation Study?: A Case Study on the O-Acetylation of Sialic Acid in Fish Serum Glycans Xin Liu and Luis Afonso 19. Bioinformatics in Glycomics: Glycan Characterization with Mass Spectrometric Data Using SimGlycan™ Arun Apte and Ningombam Sanjib Meitei

Blurb

The study of functional glycomics requires the continuous development of rapid and sensitive methods for the identification of glycan structures and integration to structure-function relationships. In Functional Glycomics: Methods and Protocols, a panel of world-renowned experts provide new developments and emerging glycomics techniques in the form of detailed protocols exploring the fundamental challenges and most cutting-edge novel applications. Written in the highly successful Methods in Molecular Biology™ series format, chapters in this volume present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting key tips on troubleshooting and avoiding known pitfalls.

Authoritative and easy to use, Functional Glycomics: Methods and Protocols serves as an ideal reference for scientists working in biochemistry, molecular biology, cell biology, immunology, microbiology, and virology and a guide to appropriate techniques and the design of achievable research plans in this vital field.