Chapter 8: Bioinformatics of functional disulphides #1
Chapter 9: Bioinformatics of functional disulphides #2
Chapter 10: Mechano-chemistry of disulphide bonds
Chapter 11: Oxidoreductase cleavage of disulphide bonds
Chapter 12: Thiol-disulphide cleavage of disulphide bonds
Chapter 13: Experimental screens for identification of functional disulphides
Chapter 14: Quantification of the redox state of functional disulphides
Chapter 15: Kinetic trapping of allosteric disulphides
Chapter 16: Identification of allosteric disulphides from labile disulphides in protein structures
Chapter 17: S-nitrosylation control of functional disulphides
Chapter 18: S-glutathionylation control of functional disulphides
Chapter 19: Reporter probes of functional disulphide bonds
Chapter 20: Measurement of subcellular redox potentials
Chapter 21: Oxidoreductase inhibitors
Chapter 22: Case study: disulphide control of transglutaminase 2
Chapter 23: Case study: disulphide control of Lon protease
Chapter 24: Case study: disulphide control of CD4 immune function
Chapter 25: Case study: disulphide control of protein kinases
Chapter 26: Case study: disulphide control of integrin function
This book describes the current techniques used to study functional disulphides and to exploit them for therapeutic outcomes. Beginning with how disulphide bonds are classified and how to study their evolution, the volume then continues with protein chemical, molecular dynamics, and force spectroscopy techniques used to identify functional disulphides and/or study their behavior, then focuses on techniques employed in the study of the factors that cleave and/or form disulphide bonds. Methods to study how particular functional disulphides are assayed in mouse and human tissues are likewise examined, amongst many other subjects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Functional Disulphide Bonds: Methods and Protocols serves as a vital guide to this evolving area of study.