Foxo Transcription Factors: Methods and Protocols

ISBN-13: 9781493988990 / Angielski / Twarda / 2018 / 270 str.

Wolfgang Link

Opis

Chapters guide readers through biochemical and molecular methods, imaging approaches to monitor the subcellular localization of FOXO factors, omics and bioinformatics approaches, different animal models used in FOXO research, and human studies to investigate the role of FOXO factors in human disease and longevity.

Spis treści

1. Introduction to FOXO Biology

Wolfgang Link

 

Part I: Biochemical and Molecular Methods

2. Characterization of microRNAs Regulating FOXO Expression

Doug Hanniford and Eva Hernando

 

3. Identification of Transcription Factor Binding Sites in the Mouse FoxO1 Promoter 

Christopher P. Cardozo

 

4. Investigating Mechanisms that control Ubiquitin-mediated DAF-16/FOXO Protein Turnover

Thomas Heimbucher and Coleen T. Murphy

 

5. Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate their Activity

Alba Orea-Soufi, David Dávila, María Salazar-Roa, María de Mar Lorente, and Guillermo Velasco

 

6. Methodological Approach for the Evaluation of FOXO as a Positive Regulator of Antioxidant Genes

María Monsalve, Ignacio Prieto, Andreza Fabro de Bem, and Yolanda Olmos

 

7. Characterization of FOXO Acetylation

Shang Yao, Zimam Mahmud, Nikoleta Sachini, Sathid Aimjongjun, Paula Saavedra-García, and Eric W-F Lam

 

8. Transcriptional Activity of FoxO Transcription Factors Measured by Luciferase Assays

Antonia Ávila-Flores, Javier Arranz-Nicolás, and Isabel Mérida

 

9. Monitoring the Transcriptional Activity of FOXO Transcription Factors by Analyzing their Target Genes

Vanessa Henriques, Susana Machado, Wolfgang Link, and Bibiana I. Ferreira

 

10. Using ChIP-based Approaches to Characterize FOXO Recruitment to its Target Promoters

Neeraj Kumar and Arnab Mukhopadhyay

 

11. RNAi Mediated Silencing of FoxO Factors

Priyankar Sanphui, Akash Saha, and Subhas Chandra Biswas

 

Part II: Imaging Approaches

12. Immunofluorescence Analysis by Confocal Microscopy for Detecting Endogenous FOXO

Francisco Castillo, Thomas A. Mackenzie,  and Bastien Cautain

 

13. High Throughput Image-based Screening to Identify Chemical Compounds Capable of Activating FOXO

Marta Barradas, Wolfgang Link, Diego Megías, and Pablo J. Fernández-Marcos

 

14. Image-based Identification of Chemical Compounds Capable of   Trapping FOXO in the Cell Nucleus

Susana Machado, Catarina Raposo, Bibiana I. Ferreira, and Wolfgang Link

 

15. Quantifying Tissue-specific Overexpression of FOXO in Drosophila via mRNA Fluorescence in situ Hybridization using Branched DNA Probe Technology

Anna C Blice-Baum, Georg Vogler, Meera C Viswanathan, Bosco Trinh, Worawan B Limpitikul, and Anthony Cammarato

 

Part III: Omics and Bioinformatic Approaches

16. Genome-wide Analysis for Identifying FOXO Protein Binding Sites

Dong-Ju Shin, Pujan Joshi, Dong-Guk Shin, and Li Wang

 

17. Mathematical Modeling of Nuclear Trafficking of  FOXO Transcription Factors

Bradford E. Peercy and Martin F. Schneider

 

18. Following Transcriptome to Uncover FOXO Biological Functions

Raymond Liang, Vijay Menon, and Saghi Ghaffari

 

Part IV: Animal Models

19. Hydra as Model to Determine the Role of FoxO in Longevity

Thomas C. G. Bosch

 

20. Genetic Ablation of FOXO in Mice to Investigate its Physiological Role

Hwanhee Oh and Jihye Paik

 

Part V: Human Gentic and Clinical Studies

21. Analysis of FOXO3 Gene Polymorphisms Associated with Human Longevity

Timothy A Donlon, Philip MC Davy, and Bradley J Willcox

 

22. Analysis of FOXO3a Gene Polymorphism Associated with Asthma

Tejas Shah and Kalpana Joshi

Blurb

This volume provides an overview of experimental procedures and state-of art methods to investigate FOXOs. Chapters guide readers through biochemical and molecular methods, imaging approaches to monitor the subcellular localization of FOXO factors, omics and bioinformatics approaches, different animal models used in FOXO research, and human studies to investigate the role of FOXO factors in human disease and longevity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge FOXO Transcription Factors: Methods and Protocols aims to ensure successful results in the further study of this vital field.