Fluorescent Proteins: Methods and Protocols

ISBN-13: 9781071626665 / Angielski / Twarda / 2022

Sharma, Mayank

Opis

This volume brings together cutting-edge laboratory protocols to characterize the novel fluorescent proteins (FPs) and approaches based on fluorescent proteins that aim to answer some of the key cell biological questions. The book covers topics ranging from the database of fluorescent proteins to their characterization and adaptation to a wide range of biological systems. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Fluorescent Proteins: Methods and Protocols serves as an ideal guide for students and academicians enthusiastic about the recent progress in the practical application of fluorescent protein technology.

Spis treści

1. Using FPbase: The Fluorescent Protein Database

            Talley J. Lambert

 

2. Selecting the Fluorescent Protein for In Vivo Imaging Experiments

            Mayank Sharma

 

3. Advanced Fluorescence Microscopy Methods to Study Dynamics of Fluorescent Proteins In Vivo

            Marcela Díaz and Leonel Malacrida

 

4. Directed Evolution of Fluorescent Proteins in Bacteria

            Sara Mattson, Geraldine N. Tran, and Erik A. Rodriguez

 

5. Fluorescent Proteins: Crystallization, Structural Determination, and Non-Natural Amino Acid Incorporation

            Rochelle D. Ahmed, Husam Sabah Auhim, Harley L. Worthy, and D. Dafydd Jones

 

6. Development and Characterization of Flavin-Binding Fluorescent Proteins, Part I: Basic Characterization

            Alina Remeeva, Anna Yudenko, Vera V. Nazarenko, Oleg Semenov, Anastasia Smolentseva, Andrey Bogorodskiy, Ivan Maslov, Valentin Borshchevskiy, and Ivan Gushchin

 

7. Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization

            Nora Lisa Bitzenhofer, Fabienne Hilgers, Gabriela N. Bosio, Joaquim Torra, Giorgia Casini, Felix R.M. Beinlich, Esther Knieps-Grünhagen, Valentin Gordeliy, Karl-Erich Jaeger, Santi Nonell, Ulrich Krauss, Thomas Gensch, and Thomas Drepper

 

8. CRISPR-Cas9-Mediated Knock-In Approach to Insert the GFP₁₁ Tag into the Genome of a Human Cell Line

            Ryo Tamura and Daichi Kamiyama

 

9. Fluorescent Protein-Based Approaches for Sub-Cellular Protein Localization in Plants

            Mayank Sharma, Ralf Bernd Klösgen, and Bationa Bennewitz

 

10. Growth Rates Evaluation of the Budding Yeast Saccharomyces cerevisiae Cells Carrying Endogenously Expressed Fluorescent Protein Fusions

            Kara L. Schneider, Lucas E. Reibenspies, Thomas Nyström, and Sviatlana Shashkova

 

11. DNA Visualization Using Fluorescent Proteins

            Xuelin Jin, Y. Tehee Kim, and Kyubong Jo

 

12. Quantification of Intracellular Citrate Concentrations with Genetically Encoded Biosensors

            Yufeng Zhao, Kei Takahashi-Yamashiro, Yi Shen, and Robert E. Campbell

 

13. Quantification of Redox-Sensitive GFP Cysteine Redox State via Gel-Based Read-Out

            Finja Bohle, Andreas J. Meyer, and Stefanie J. Mueller-Schuessele

 

14. Assessing In Vivo Oxygen Dynamics Using Plant N-Terminal Degrons in Saccharomyces cerevisiae

            Mikel Lavilla-Puerta and Beatrice Giuntoli

 

15. Osmium Resistant Fluorescent Proteins and In-Resin Correlative Light-Electron Microscopy of Epon-Embedded Mammalian Cultured Cells

            Isei Tanida, Junji Yamaguchi, Soichiro Kakuta, and Yasuo Uchiyama

 

16. Fluorescence-Detection Size-Exclusion Chromatography-Based Thermostability Assay for Membrane Proteins

            Hebang Yao, Hongmin Cai, and Dianfan Li

 

17. Determining Protein-Protein Interaction with GFP-Trap Beads

            Irem Yilmazer, Melanie R. Abt, Yuanyuan Liang, David Seung, Samuel C. Zeeman, and Mayank Sharma